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This retrospective case-control study to assess the impact of Cyclin D1 and CDK4 expression along with EBV infection with Hodgkin’s lymphoma (HL).80 lymph node included in this study; (40) formalin-fixed, paraffin- embedded biopsies from Hodgkin’s lymphoma (HL), and (40) apparently healthy lymph nodes as control group. EBV detection was done by CISH whereas IHC detection system was used to evaluate both Cyclin D1 & CDK4 gene expression. The positive –EBERs CISH reactions detected in 16 / 40 (40 %) of Hodgkin lymphoma tissues while positive - EBERs reactions detected in 3 out of 40 tissues (7.5%) in control lymph nodes tissues. Statistically was found highly significant between study groups (P value = < 0.05). Cyclin D1 & CDK4 -IHC were detected in 40% and 52.5% of HL, respectively. Statistically, significant differences noticed among negative, low and moderate scoring of tissues (P<0.05). Significant EBV gene expressions along with higher percentage of tissues in which Cyclin D1 & CDK4 genes being expressed may be indicator their possible role in the pathogenesis and / or the carcinogenesis of a subset of our Hodgkin’s lymphoma cases. [ABSTRACT FROM AUTHOR] more...

Background: Viral, dietary and genetic factors are implicated in the etiology ofnasopharyngeal cancer, a rare type of head and neck cancers. Unlike other viruses, HHV-8 encodes several human cytokines homologues and regulatory genes that play important roles in the viral pathogenesis. Objective: To analyze the rates of HHV-8 infection in tissues obtained from a group of patients with nasopharyngeal carcinoma and inflammatory nasal polyps (INP) . Patients and Method: One hundred- thirty formalin-fixed, paraffin- embedded nasopharyngeal carcinoma and nasal inflammatory polyps tissues enrolled in this study; 65 nasopharyngeal tissue biopsies from nasopharyngeal carcinoma; 35 tissue biopsies from nasal inflammatory polyps and 30 nasopharyngeal tissues with unremarkable pathological changes, as apparently healthy tissuecontrol. Detection of HHV-8 was done by chromogenic in situ hybridization (CISH) technique detection system. Results: In nasopharyngeal carcinoma tissues, the HHV-8- DNA positive CISH reactions were detected in 23.1% while in nasal inflammatory polyps tissues HHV-8-positive CISH reactions were found in 8.6% of the examined tissues. The correlation between HHV-8 and NPC & INP was highly significant (P= 0.001). Conclusion: Significant HHV-8 detection in nasopharyngeal carcinoma & inflammatory nasal polypstissues could point for their possible role in either pathogenesis or carcinogenesis of both these lesions. [ABSTRACT FROM AUTHOR] more...