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Your search keyword '"de la Rosa FF"' showing total 2 results
Start Over Author "de la Rosa FF" Publisher instituto espanol de fisiologia y bioquimica
2 results on '"de la Rosa FF"'

1. Studies on the kinetic mechanism of nitrate reductase from spinach (Spinacea oleracea).

Author

de la Rosa FF, Palacián E, and Castillo F

Subjects
Kinetics, NADH, NADPH Oxidoreductases physiology, Nitrite Reductases physiology, Vegetables
Abstract

Based on Lineweaver-Burk plots of the initial velocities, at different concentrations of NADH and nitrate, and product inhibition patterns, an Iso Ping Pong Bi Bi steady state kinetic mechanism is proposed for the spinach nitrate reductase. This mechanism incorporates the concept that the oxidized enzyme is present in two isomeric forms.

Published
1980

2. Nitrate reductase from Spinacea oleracea. FAD and the reactivation of the enzyme treated with p-Hydroxymercuribenzoate.

Author

Castillo F, de la Rosa FF, and Palacián E

Subjects
Dihydrolipoamide Dehydrogenase analysis, Enzyme Reactivators, Mercuribenzoates pharmacology, Flavin-Adenine Dinucleotide analysis, Nitrate Reductases analysis, Vegetables analysis
Abstract

Spinach nitrate reductase complex previously inactivated by treatment with mercurials p-hydroxymercuribenzoate or p-hydroxymercuriphenyl sulphonate can be reactivated by incubation with dithioerythritol. The reactivation of NADH-diaphorase seems to be FAD-dependent, whereas that of FNH2-nitrate reductase is not. The requirement of FAD for NADH-inactivation of nitrate reductase treated with p-hydroxymercuribenzoate disappears after treatment with dithioerythritol.

Published
1977

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