1. [Growth and survival of total and pathogenic Vibrio parahaemolyticus in American oyster (Crassostrea virginica) under cold storage].
- Author
-
Flores-Primo A, Pardío-Sedas VT, López-Hernández K, Lizárraga-Partida L, and Uscanga-Serrano R
- Subjects
- Animals, Bacterial Load, Cold Temperature, Gene Expression Regulation, Bacterial, Genes, Bacterial, Maximum Allowable Concentration, Mexico, Seasons, Vibrio parahaemolyticus growth & development, Vibrio parahaemolyticus isolation & purification, Vibrio parahaemolyticus pathogenicity, Virulence genetics, Crassostrea microbiology, Food Microbiology, Food Preservation methods, Food Storage methods, Refrigeration, Shellfish microbiology, Vibrio parahaemolyticus physiology
- Abstract
Objective: To quantify Vibrio parahaemolyticus densities in American oyster (Crassostrea virginica) under cold storage., Materials and Methods: 320 oysters were stored at 7°C for nine days and total and pathogenic densities were determined by the NMP-PCR methodology., Results: V. parahaemolyticus tlh+ densities were observed on 0,3, and 6 days of storage at 1.134, 2.764 and 0.785 log10NMP/g, respectively, and pathogenic density trh+ on 0 and 3 days at 0.477 and 0.519 log10NMP/g, respectively; the pathogenic densities tdh+ (0.519 log10NMP/g), tdh+/trh+ (0.519 log10NMP/g), and tdh+orf8+ (-0.444 log10NMP/g) were detected on day 3 of storage., Conclusion: The results suggest that V. parahaemolyticus growth and pathogenic genes occurrence at 7°C involve changes in the genetic expression as a cold shock response, favoring V. parahaemolyticus survival and virulence, representing a health risk.
- Published
- 2015