1. Insights into the proteomic profile and gene expression of Lutzomyia longipalpis-derived Lulo cell line
- Author
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Roger Magno Macedo Silva, Franklin Souza da Silva, Bernardo Acácio Santini Pereira, Otacilio C. Moreira, Priscila Silva Grijó Farani, Mariana Caldas Waghabi, Daniela de Pita-Pereira, Leila Mendonça-Lima, Carlos Roberto Alves, Luzia Monteiro de Castro Côrtes, Geovane Dias-Lopes, Felio J. Bello, Paloma Resende Corrêa, and Suzana Corte-Real
- Subjects
Proteomics ,Microbiology (medical) ,In silico ,030231 tropical medicine ,RC955-962 ,Lulo cell ,Microbiology ,Leishmania braziliensis ,Cell Line ,03 medical and health sciences ,0302 clinical medicine ,Arctic medicine. Tropical medicine ,Gene expression ,Animals ,Gene ,Leishmania (Viannia) braziliensis ,proteomic ,Leishmania ,Proteomic Profile ,biology ,RT-qPCR ,biology.organism_classification ,Molecular biology ,QR1-502 ,Membrane protein ,Vacuolar transport ,Original Article ,Psychodidae ,Transcriptome - Abstract
BACKGROUND Lutzomyia longipalpis-derived cell line (Lulo) has been suggested as a model for studies of interaction between sandflies and Leishmania. OBJECTIVES Here, we present data of proteomic and gene expression analyses of Lulo cell related to interactions with Leishmania (Viannia) braziliensis. METHODS Lulo cell protein extracts were analysed through a combination of two-dimensional gel electrophoresis and mass spectrometry and resulting spots were further investigated in silico to identify proteins using Mascot search and, afterwards, resulting sequences were applied for analysis with VectorBase. RESULTS Sixty-four spots were identified showing similarities to other proteins registered in the databases and could be classified according to their biological function, such as ion-binding proteins (23%), proteases (14%), cytoskeletal proteins (11%) and interactive membrane proteins (9.5%). Effects of interaction with L. (V.) braziliensis with the expression of three genes (enolase, tubulin and vacuolar transport protein) were observed after an eight-hour timeframe and compared to culture without parasites, and demonstrated the impact of parasite interaction with the expression of the following genes: LLOJ000219 (1.69-fold), LLOJ000326 (1.43-fold) and LLOJ006663 (2.41-fold). CONCLUSIONS This set of results adds relevant information regarding the usefulness of the Lulo cell line for studies with Leishmania parasites that indicate variations of protein expression.