1. Detection of hepatitis C virus RNA by in situ hybridization in paraformaldehyde fixed biopsies.
- Author
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Carvalho HS, Baptista ML, Pinto MA, Silva M, Takiya CM, Chagas V, Pannain V, Coelho HS, and Yoshida CF
- Subjects
- Adult, Aged, Alanine Transaminase blood, Biopsy, Digoxigenin, Female, Formaldehyde, Genotype, Hepacivirus isolation & purification, Hepatitis C, Chronic pathology, Humans, Liver pathology, Male, Middle Aged, Paraffin Embedding, Polymorphism, Restriction Fragment Length, RNA, Viral genetics, Reverse Transcriptase Polymerase Chain Reaction, Severity of Illness Index, Hepacivirus genetics, Hepatitis C, Chronic virology, In Situ Hybridization methods, Liver virology, RNA, Viral isolation & purification
- Abstract
Fourteen hepatitis C virus (HCV) chronically infected patients were submitted to routine liver biopsy for histological evaluation. Liver samples were assayed to HCV-RNA by in situ hybridization, using digoxigenin labeled probe. HCV genotypes were found to be predominantly type 1 (71.4%), followed by genotype 3 (21.4%), and genotype 2 (7.2%). Alanine-aminotransferase levels were raised in 10 patients. The histopathological scores were minimal (21.4%), mild (57.2%), and moderate (21.4%). Viral RNA was detected in liver cells from nine patients (64.3%). ISH method provides localization and poor confirmation of HCV RNA in the liver tissue of HCV chronic patients.
- Published
- 2005
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