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Introduction: Trigona thoracica propolis is known to have antimicrobial properties, however its antileptospiral properties and its synergistic effects with commonly prescribed antibiotics are scarcely documented. This study aimed to evaluate the antileptospiral properties of Trigona thoracica against pathogenic Leptospira species (spp.) and to study its synergistic effects with commonly prescribed antibiotics. Materials and Methods: The tested Leptospira serovars were Australis, Bataviae, Canicola and Javanica. Aqueous extract propolis (AEP) and ethanolic extracts propolis (EEP) were used. Broth dilution methods were used to determine the Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC) and the synergistic effects between the propolis and the tested antibiotics. The synergistic effects was evaluated by using the fractional inhibitory concentration (FIC) index. Morphological changes of the treated Leptospira were observed under a Scanning Electron Microscope (SEM). Results: The AEP and EEP were found to have antileptospiral properties against the tested Leptospira spp. The synergy result showed that only combination of AEP and penicillin G against serovar Australis has demonstrated synergistic effect with the FIC index of 0.38. Morphological study using SEM showed significant structural changes of the treated Leptospira spp. Conclusions: The result suggests that Trigona thoracica propolis could potentially be used as either a complimentary or an alternative therapeutic agent against pathogenic Leptospira spp. [ABSTRACT FROM AUTHOR]

Hydroxychavicol (HC) is a phenolic compound of betel leaf (Piper betle). It has been reported to have antifungal properties against dermatophytes including T. rubrum. The aim of this study was to identify the effects of the HC against T. rubrum. Broth dilution method was used to determine the minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of the HC. Microscopic study of the treated fungus was done by transmission electron microscope (TEM). Cytotoxicity study using pre-adipocyte (3T3-L1) cell line was performed by means of MTT cell proliferation assay. The MIC and MFC results of the HC were both 0.49 µg/ml. Microscopic study revealed the destruction of the fungal cell wall and organelles. Cytotoxicity study showed HC to be non-toxic to the tested human cell line. In conclusion, HC may potentially be used as an alternative therapeutic agent against T. rubrum infections. [ABSTRACT FROM AUTHOR]

Introduction: Trichophyton rubrum is a common pathogenic fungal species that is responsible for causing infection of human skin, hair and nail. The antifungal-resistant strains however, complicate the treatment regime. Hydroxychavicol (HC) is one of the main compounds from the Piper betel leaf that have antifungal potential and its mechanism of action has yet to be studied. This study was carried out to determine the antifungal properties of HC against Trichophyton rubrum using transmission electron microscope (TEM) on gross and ultrastructure of Trichophyton rubrum hypha. Methods: Broth dilution method was used to determine the minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of HC and miconazole (MI) against the Trichophyton rubrum (ATCC 28188). Trichophyton rubrum was treated with HC and MI at concentrations of 1.25, 2.5, 5 and 10 mg/mL for 1, 3, 5 and 7 days continuously. Results: The MIC results of the HC and MI against Trichophyton rubrum were 0.00048 mg/mL and 0.000061 mg/mL respectively. MFC results showed 0.0019 mg/mL for HC and 0.000061 mg/mL for MI. Microscopically, the fungi structures became more severely damaged at increasing treatment duration. The cell wall of the fungi treated with HC showed a rough surface, shrinkage and demolition similar to that of the MI treated group. The fungi organelles were also demolished and disorganized. Conclusions: This study reveals that HC has the ability to inhibit Trichophyton rubrum growth and it has the potential to be an antifungal agent especially in treating dermatitis. [ABSTRACT FROM AUTHOR]