1. Effect of polydiacetylene-based nanosomes on cell viability and endocytosis.
- Author
-
Park KH, Ku M, Yoon N, Hwang DY, Lee J, Yang J, and Seo S
- Subjects
- Cell Line, Tumor, Humans, Liposomes administration & dosage, Liposomes chemistry, Nanoparticles chemistry, Polyacetylene Polymer chemistry, Cell Survival drug effects, Endocytosis drug effects, Nanoparticles administration & dosage, Polyacetylene Polymer administration & dosage
- Abstract
Polydiacetylene-based nanoparticles have been developed as nanocarriers for various bio-applications. However, how nanocarriers enter the cell environment and affect cell viability has not yet been considerably explored. In this study, polydiacetylene-based nanoliposomes (nanosomes) were electrostatically complexed with rhodamine fluorophores. Based on real-time cell imaging and cell viability assessment, the most highly polymerized nanosomes were found to be less toxic to cells. Moreover, it was revealed that the rhodamine/polydiacetylene nanosome complex dissociates at cell environment, the polydiacetylene nanosome penetrates into cells, as suggested by the fluorescence observed in confocal microscopy images.
- Published
- 2019
- Full Text
- View/download PDF