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In this experiment, 'Jinyan' kiwifruit field leaves were used as explants for tissue culture to study the effects of different plant growth regulators on the regeneration of leaves of 'Jinyan' kiwifruit, including the induction of adventitious shoots, the proliferation of adventitious shoots and the rooting of adventitious buds. Establish a rapid propagation system for 'Jinyan' kiwifruit. The results showed that the optimal medium for the regeneration of adventitious shoots of 'Jinyan' kiwifruit leaves was MS + 6-BA 4.0 mgL−1+NAA 0.1 mgL−1, the budding rate was 76.67%; and the best medium for adventitious shoots proliferation was MS+6-BA 2.0 mgL−1+NAA 0.4 mgL−1+GA3 0.1 mgL−1, the average proliferation coefficient was 3.33; the optimal rooting medium was 1/2MS +IBA 0.6 mgL−1, the rooting rate was 86.67%, and the average root length was 2.30 cm.