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The article analyzes the influence about backwater side sand piling of a Pile-based frame Seawall along the east coast of Zhejiang on the stability and deformation. When sand is not piled on the backwater side of the Seawall, the overall safety factor of the Seawall is 1.619∼2.941, which meets the specifications and design requirements; when the backwater side is piled with sand or sand piling+vehicle driving, the overall safety factor of the Seawall is respectively 1.119∼1.617, 1.107∼1.599, and the safety factor of some working conditions is less than required 1.20, which does not meet the specifications and design requirements. According to the simulation results of the MIDAS finite element software, the settlement of the Seawall within 3 months of sand piling is 22.0mm, and the horizontal displacement is 7.9mm, indicating that the sand piling on the back side has a great influence on the deformation of the Seawall. The settlement and horizontal displacement of the Seawall are too large, resulting in a large reduction in the vertical and horizontal bearing capacity of the pile foundation, which has a huge impact on the overall stability and safe operation, severely disrupting the stress balance of the Seawall structure, and causing landslides on the water-facing side risk.

The article analyzes the impact of a constructing sewage treatment plant on the built seawall nearly by PLAXIS finite element software, located on the coast eastern Zhejiang. As the construction carrying on, the settlement and horizontal displacement are gradually increasing on the top and backwater slope of the seawall. The settlement on the top of Seawalls is 35.91 cm, the horizontal displacement is 50.76 cm; the settlement on the back slope is 71.45 cm, the horizontal displacement is 48.54cm. During the construction process, settlement increase rate at the foot of the backwater slope was significantly larger than that at the top of the seawalls. The horizontal displacement increase rate at the top of the seawalls was slightly larger than that at the foot of the backwater slope. The settlement and horizontal displacement of the seawall exceed the normal allowable deformation, which threatens the safety of the seawall. It is necessary to take reinforcement measures for the seawall to ensure safe operation.

Low-energy ion beam implantation (10 ~ 200 keV) has been proved to have a wide range of biological effects and is broadly used in the breeding of crops and micro-organisms.To understand its mechanisms better and facilitate its applications, the developments in the bioeffects of low energy ion beam implantation in the past twenty years are summarized in this paper.

Mutant strains of GO112 and BM302 with a high 2-keto-L-gulonic acid (2KLG) transformation rate induced by ion beam implantation were separately and combinatorially compared with the original strains GO29 and BM80 to study the mutagenic effects of ion beam implantation. Both the sole GO112 and mixed BM302:GO112 demonstrated improved SNDH activity and 2KLG yield compared to the original strains. The mutant combinations of BM302:GO112 showed a longer stationary phase and higher biomass than BM80:GO29. The mutant BM302 exhibited a stronger capacity to maintain a stable pH environment at mixed fermentation with Gluconobacter oxydans (G. oxydans) for 2KLG transformation and facilitated the growth of G. oxydans compared with the original strain BM80. The promotive capacity to L-sorbosone dehydrogenase (L-SNDH) from the supernate of BM302 was 1.6-fold higher than that of BM80. Genes encoded SNDH in GO29 and GO112 were amplified and sequenced, and mutations including three transitions (CG → TA, CG → TA, GC → AT) and one transversion (AT → TA) were confirmed from GO29 to GO112. The corresponding amino acid was changed as Leu → Phe, Arg → Gln and Asn → Lys.