1. Identifying and quantifying the stromal fibrosis in muscularis propria of colorectal carcinoma by multiphoton microscopy
- Author
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Yinghong Yang, Weizhong Jiang, Xiaoqin Zhu, Changyin Feng, Shuangmu Zhuo, Sijia Chen, Guoxian Guan, Zhifen Chen, and Jianxin Chen
- Subjects
Pathology ,medicine.medical_specialty ,Stromal cell ,Diagnostic methods ,Physics and Astronomy (miscellaneous) ,Colorectal cancer ,business.industry ,medicine.disease ,Multiphoton fluorescence microscope ,Stroma ,Fibrosis ,medicine ,business ,Instrumentation ,Pathological ,Infiltration (medical) - Abstract
The examination of stromal fibrosis within colorectal cancer is overlooked, not only because the routine pathological examinations seem to focus more on tumour staging and precise surgical margins, but also because of the lack of efficient diagnostic methods. Multiphoton microscopy (MPM) can be used to study the muscularis stroma of normal and colorectal carcinoma tissue at the molecular level. In this work, we attempt to show the feasibility of MPM for discerning the microstructure of the normal human rectal muscle layer and fibrosis colorectal carcinoma tissue practicably. Three types of muscularis propria stromal fibrosis beneath the colorectal cancer infiltration were first observed through the MPM imaging system by providing intercellular microstructural details in fresh, unstained tissue samples. Our approach also presents the capability of quantifying the extent of stromal fibrosis from both amount and orientation of collagen, which may further characterize the severity of fibrosis. By comparing with the pathology analysis, these results show that the MPM has potential advantages in becoming a histological tool for detecting the stromal fibrosis and collecting prognosis evidence, which may guide subsequent therapy procedures for patients into good prognosis.
- Published
- 2014