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There is a critical need to developin vitroculture systems appropriate for the expansion of adipose tissue, in order to gain new insights into metabolic diseases and to assist in the restoration of tissue defects. Conventional two- or three-dimensional (2D or 3D)in vitromodels of adipocytes require a combination of supplements to induce adipocyte maturation that greatly increases the cost of large-scale industrial production. In the present study, a microporous, perforated bacterial cellulose (BC)-assisted culture system was developed that promoted the adhesion, proliferation, and adipogenic differentiation of preadipocytes. Additionally, the system maintained the cells as mature unilocular adipocytesex vivoin normal cell culture medium in long-term culture. All cells were derived from isolated adipose tissue without the use of expensive enzymes for tissue digestion. In contrast to culture in hard tissue culture plates, preadipocytes in the soft 3D environments formed multidimensional interlaced cell contacts, undergoing significant spontaneous lipid accumulation and could be cultured for up to threemonths in maintenance medium. More importantly, the cultured adipose tissue-derived cell bank created here was able to produce injury repair activators that promoted the proliferation of fibroblasts with little fibrosis and the functional differentiation of myoblasts, displaying the potential for use in adipose reconstruction. Thus, the present study demonstrates the potential of a mechanically flexible BC scaffold to generate volume tunable adipose constructs and provides a low-cost and user-friendly strategy for large-scale industrial production of adipose tissue.

The traditional recommendation system can hardly utilize the ingredients and flavor characteristics of the dishes, and faces the problems of sparse data and cold start, resulting in inaccurate results of the recommendation system. This paper addresses these problems by constructing a heterogeneous graph network with the interaction data between users and dishes in the food domain and taking the main and auxiliary ingredients as nodes. Also, we capture the higher-order structural information among users, dishes, and main and auxiliary ingredients by using meta-path guidance. In the meantime, we assign weights to the edges associated with user nodes and main and auxiliary ingredients nodes, which can obtain users’ preferences for main and auxiliary ingredients by using weighted GCN networks. The experiments conducted on a food domain dataset demonstrate that the meta-path-guided heterogeneous graph dish recommendation algorithm proposed in this paper is improved over the traditional recommendation algorithm.

Mechanically strengthened, highly cube textured Ni?7?at.%W/Ni?12?at.%W multi-layer substrates developed for coated conductors have been prepared by the advanced spark plasma sintering technique. The key innovation for developing this weakly magnetic and reinforced substrate was to use a new powder metallurgy and sintering route to bond multi-layers of Ni7W/Ni12W/Ni7W together in order to get an initial ingot, followed by the optimized cold working and annealing. Particular efforts were made in view of the optimization of the design, pressing as well as the heat treatment processes of the starting ingots to obtain a chemically gradient composite bulk, thus ensuring the subsequent cold deformation. The produced composite substrates have a strong {100} texture on Ni7W outer layers. The percentage of the biaxially orientated grains within a misorientation angle of 10? is as high as 97.5%, while the length percentage of low-angle grain boundaries ranging from 2? to 10? in the composite substrate reaches 87.2%. Moreover, the yield strength ?0.2 of the tape approaches 333?MPa, and the saturation magnetization is substantially reduced by 81.6% at 77?K when compared to that of a commercial used Ni5W substrate.

Mechanically strengthened, highly cube textured Ni-7at.%W/Ni-12at.%W multi-layer substrates used for coated conductors have been prepared by advanced spark plasma sintering technique. The key innovation for developing this weakly magnetic and reinforced substrate was to use a new powder metallurgy and sintering route to bond multi-layers of Ni7W-Ni12W-Ni7W together in order to get an initial ingot, then followed by optimized cold working and annealing. Particular efforts were made in view of the optimization of the design, pressing as well as the heat treatment processes of the starting ingots in order to obtain a chemically gradient composite bulk, thus ensuring the subsequent cold deformation of the bulk. The produced composite substrates have a strong {100} texture on the top Ni7W outer layer determined by EBSD and X-ray. The percentage of the biaxially orientated grains within a misorientation angle of 10° is as high as 97.5%, while the length percentage of low angle GBs ranging from 2° to 10° in the composite substrate reaches 87.2%. Moreover, the yield strength σ0.2 of the tape approaches 333 MPa, and the saturation magnetization is substantially reduced by 81.6% at 77K when compared to that of a commercial used Ni5W substrate.

We report on the development of mechanically strengthened, highly textured Ni–5 at.%W/Ni–12 at.%W composite materials prepared by a powder metallurgical approach as promising weakly magnetic substrates for coated superconductors. The key configuration of this composite substrate consists of a thin, sharp cubic textured Ni–5 at.%W layer on a Ni–12 at.%W alloy core, thus providing a mechanical reinforcement while decreasing the saturation magnetization of the whole substrate. The composite substrates have a sharp cubic texture at the top Ni–5 at.%W outer layer and their yield strength reaches 272 MPa, exceeding that of the commercially used Ni5W substrates by a factor of 1.6. The saturation magnetization of the composite substrate Ni5W/Ni12W/Ni5W is substantially reduced when compared to that of pure Ni and Ni–5 at.%W substrates, respectively.