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Introduction: Although micro-computed tomography (MCT) evaluation of the shaping ability of ProTaper Next (PTN) and Hyflex CM (HCM) files has been reported, to our knowledge, no study has assessed the performance of V-Taper 2H (VT) files. The aim of this study was to evaluate and compare the shaping ability of PTN, HCM, and VT systems in the mesial canals of mandibular molars using MCT., Methods and Materials: Thirty extracted first and second mandibular molars were scanned using MCT and randomly assigned to HCM, PTN, and VT groups. Images obtained before and after preparation were evaluated for the increase in the root canal volume, untouched surface area, and amount of accumulated hard tissue debris. One-way analysis of variance (ANOVA) and Kruskal-Wallis test were used to compare the variables in the groups ( α =5%)., Results: There were no statistically significant between-group differences in the postoperative measurements ( P >0.05). The canal volume increased in all three groups: PTN (73.84%), VT (73.48%), and HCM (49.29%). The largest and smallest untouched areas were observed in the PTN (41.37%) and VT (30.85%) groups, respectively ( P >0.05). The debris formed during canal preparation was 1.84%, 2.16%, and 2.42% in the VT, PTN, and HCM groups, respectively ( P >0.05)., Conclusions: Based on our in vitro study, the PTN, HCM, and VT systems showed similar shaping abilities. None of the tested canals were completely free from debris, while the untouched surface area was considerably large. The VT system had the most favorable results with the smallest untouched surface area and least debris were. We would recommend further trials to endorse these findings., Competing Interests: ‘None declared’., (© The Author(s).)