1. A novel GlcNAcalpha1-HPO3-6Gal(1-1)ceramide antigen and alkylated inositol-phosphoglycerolipids expressed by the liver fluke Fasciola hepatica.
- Author
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Wuhrer M, Grimm C, Zahringer U, Dennis RD, Berkefeld CM, Idris MA, and Geyer R
- Subjects
- Animals, Antibodies, Helminth blood, Antigens, Helminth analysis, Antigens, Helminth chemistry, Ceramides chemistry, Ceramides immunology, Chromatography, Gas methods, Chromatography, High Pressure Liquid methods, Enzyme-Linked Immunosorbent Assay methods, Fasciola hepatica immunology, Gas Chromatography-Mass Spectrometry methods, Humans, Hydrofluoric Acid chemistry, Magnetic Resonance Spectroscopy methods, Phosphatidylinositols chemistry, Sheep, Spectrometry, Mass, Electrospray Ionization methods, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization methods, Ceramides analysis, Fasciola hepatica chemistry, Phosphatidylinositols analysis
- Abstract
The acidic (glyco)lipids of the parasitic liver fluke Fasciola hepatica exhibited two different phosphate-containing species, designated AL-I and AL-II, which were analyzed by MALDI-TOF MS, ESI MS, NMR, methylation analysis, and combined GC-MS in conjunction with HF treatment. AL-I was structurally determined as 1-O-hexadecyl-sn-glycerol-3-phosphoinositol, an ether bond variant of lysophosphatidylinositol. The structure of AL-II was shown to be GlcNAcalpha1-HPO3-6Gal(1-1)ceramide. Ceramide analysis revealed as major components 2-hydroxyoctadecanoic acid [18:0(2-OH)] together with C18- and C20-phytosphingosines. AL-II was apparently highly antigenic and strongly recognized by both animal- and human-F. hepatica infection sera. Furthermore, inhibition ELISAs revealed that the unusual antigenic determinant GlcNAcalpha1-HPO3- phosphate might have a potential in the serodiagnosis of F. hepatica infections.
- Published
- 2003
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