1. Outer Membrane Protein A (OmpA) Conferred Immunoprotection against Enterobacteriaceae Infection in Mice.
- Author
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R. Hu, Z.Y. Fan, H. Zhang, C.Y. Tong, J.Q. Chi, N. Wang, R.T. Li, L. Chen, Z.F. Ding, L.X. Chen, W. Tang, X. Zhou, L.J. Pu, Z.B. Zhu, and Y.D. Cui
- Subjects
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MEMBRANE proteins , *ENTEROBACTERIACEAE diseases , *LABORATORY mice , *WESTERN immunoblotting , *RECOMBINANT antibodies - Abstract
In order to investigate the protective effect of outer membrane protein A (OmpA) against Enterobacteriaceae infection in mice, ompA gene was cloned from bovine mastitis E. coli 308-2 isolate, and then the recombinant OmpA protein was expressed and purified. SDS-PAGE detected recombinant OmpA protein and Western blotting confirmed that the protein had an average molecular weight of 60 kDa. Immunological analysis indicated that OmpA protein induced high level of antibodies, IFN-γ and IL-4 cytokines. Moreover, OmpA protein not only conferred a high level of immunogenicity to protect the immunized mice against the challenge of E. coli, but also generated protection against Klebsiella pneumonia and Shigella flexneri. From the data generated during the study it was suggested that OmpA could be selected as a potential candidate for vaccine against Enterobacteriaceae. [ABSTRACT FROM AUTHOR]
- Published
- 2013