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Wood quality results from the interaction between the genotype of trees and the silvicultural conditions to which they were subjected. Based on this interaction, research on the factors that add value to the woody raw material has an impact on the production of various forest products. This study aimed to evaluate the influence of different planting spacings (3×1, 3×2, 3×3 and 3×4 m) on the wood properties of three 4-year-old Eucalyptus clones. The wood of each clone was evaluated based on basic density, dry mass, anatomy, structural chemical composition, ash content and higher heating value. For at least one of the three clones studied, the planting spacing explained the variations in the basic density, dry mass, diameter and frequency of vessels and wall thickness of the wood fibers. Regardless of the eucalyptus clone, the area of vessels, the length and fraction of the fiber wall, as well as the contents of extractives, lignin, ash and the higher heating value of the wood were not influenced by the planting spacing. In summary, the change in the useful area alters the properties of wood in eucalyptus genetic materials in different ways and intensities at 4 years old. [ABSTRACT FROM AUTHOR]