1. An [18F]-Positron-Emitting, Fluorescent, Cerebrospinal Fluid Probe for Imaging Damage to the Brain and Spine
- Author
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Richard Ting, Omer Aras, Bin He, Harikrishna Kommidi, Nandi Chen, Do Hyun Kim, Hua Guo, and Amy P. Wu
- Subjects
Intracerebral hemorrhage ,Fluorophore ,medicine.diagnostic_test ,business.industry ,Traumatic brain injury ,Medicine (miscellaneous) ,02 engineering and technology ,021001 nanoscience & nanotechnology ,medicine.disease ,030226 pharmacology & pharmacy ,3. Good health ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Cerebrospinal fluid ,Positron ,chemistry ,Positron emission tomography ,Medicine ,Fluorescein ,0210 nano-technology ,business ,Nuclear medicine ,Pharmacology, Toxicology and Pharmaceutics (miscellaneous) ,Myelography - Abstract
Fluorescein is modified to bear 18F so that it can act as both a positron emitter, and a fluorophore, allowing detection by positron emission tomography (PET), scintillation, and fluorescent imaging (FL). [18F]-2 is injected into the intrathecal space of rats and used to observe the cerebrospinal fluid (CSF) that bathes the brain and spine. Injury in three different applications is visualized with [18F]-2: 1) detection of a 0.7 mm paranasal-sinus CSF leak (CSFL); 2) detection of 0.5 mm puncture damage to the thoracic spine (acute spinal cord injury); and 3) detection of intracerebral hemorrhage/edema because of traumatic brain injury. In all models, the location of injury is visualized with [18F]-2 at high resolution. [18F]-2 PET imaging may be a superior alternative to current clinical contrast myelography and 131I, 111In or 99mTc radionuclide cisternography. Like fluorescein, [18F]-2 may also have other uses in diagnostic or fluorescence guided medicine.
- Published
- 2017