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The non-steroidal antiinflammatory drug sodium salicylate was studied for effects on the function of Types II and III glycocorticoid receptors of hepatic cytosol in female Wistar rats weighing 180-200 g. Scatchard and other analyses established sodium salicylate (12.5-50.0 mM)-induced reductions in the density of Type II glucocorticoid receptors and the association constant of the 3H-acetonide triamcinolone (Type II)-glucocorticoid receptor complex. The function of Type II glucocorticoid receptors was found to be inhibited by sodium salicylate in an non-concurrent way. Sodium salicylate (0.62-10.0 mM) enhanced the density of Type III glucocorticoid receptors. Depending on the dosage, sodium salicylate repeatedly increased the corticosterone-binding function of Type III glucocorticoid receptors. With this, the association constant of the complex of 3H-corticosterone and Type III glucocorticoid receptor substantially unchanged. The sodium salicylate stimulation of the function of Type III glucocorticoid receptors did not occur concurrently. The findings suggest that the non-steroidal antiinflammatory drug sodium salicylate has a great influence on the most important regulatory system of homeostasis--the glucocorticoid function which is a determinant in the pathogenesis of an inflammatory process.

Experiments on 78 adult rats were made to study the effects of acetylsalicylic acid and sodium salicylate administered daily for 7 and 14 days on the content of pyruvic, lactic, citric acids and inorganic phosphate in the wall of blood vessels of different function. Salicylates were demonstrated to reduce the content of citric acid by the 7th day and that of pyruvic acid by the 14th day, to increase the level of lactate and inorganic phosphorus during both observation periods. The degree of the changes revealed is unequal in different vessels, being more pronounced after sodium salicylate administration.

The effect of sodium salicylate (100 mg/kg intravenously) on the circulation rate of the cerebral blood flow, total arterial pressure, oxygen tension in the cerebral tissue (by the polarographic method) and on the pressure in the brain venous vessels was studied on anesthetized cats with controlled respiration. In rheumatic patients the action of the drug (10 ml of a 10% solution, intravenously) on the rheoencephalographic findings and the total arterial pressure was investigated. Sodium salicylate was shown to raise the tonicity of the intra- and extracranial vessels, to lower the amplitude of the pulse fluctuations and to highten the total arterial pressure. The cerebral circulation depends upon changes in the tonicity of the cerebral vessels and on the total arterial pressure, i. e. with a significant rise of the latter it increases in spite of a greater vascular resistance. Changes in the blood flow are followed by corresponding variations in the PO2 of the brain tissue. No significant changes of the pressure in the venous system of the brain could be established.

The influence exerted by a number of non-steroid antiphlogistic agents on the fermentative mechanism responsible for the formation and decomposition of kininines in an inflammation focus and on the permeability of vessels in a skin changed under the effect of exogenous bradykininine was studied. Bradykininine, chigamin, sodium salicylate and mephenaminate are shown to inhibit "in vitro" the kininogenic and intensify the kininase activity of the exudate in rats with turpentine-induced pleuritis, while indometacin affects only the activity of kininogenases. Butadion, chingamin and sodium mephenaminate merely reduce the reactivity of the skin with respect to exogenous bradykininine.

In mice immunized with sheep erythrocytes introduction of acetylsalycilic acid (250 mg/kg), sodium salicylate (100 mg/kg) and mephenamic acid (100 mg/kg) exerted an inhibitory effect on the synthesis of antibody-forming cells and the titre of hemagglutinines in immunized animals. The acetylsalicylic acid and sodium salicylate are more effective on the 3--5th post-immunization day; the maximal effect of the mephenamic acid becomes apparent on the 7th day and its action is more lasting.