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Your search keyword '"Turchinskiĭ MF"' showing total 3 results
Start Over Author "Turchinskiĭ MF" Publisher izdatelstvo nauka
3 results on '"Turchinskiĭ MF"'

1. [II. Use of antibodies to DNA modified by trans-diaminodichloroplatinum, for identification of specific DNA sequences].

Author

Kiseleva VI, Turchinskiĭ MF, and Poverennyĭ AM

Subjects
Animals, Base Sequence, Cattle, DNA drug effects, Immunoenzyme Techniques, Nucleic Acid Hybridization, Plasmids, Stereoisomerism, Thymus Gland metabolism, Antibodies immunology, Cisplatin pharmacology, DNA immunology
Abstract

DNA modified by trans diaminedichlorplatinum-II (transDDP) has been suggested as an effective probe for non-isotopic hybridization and high-specific anti-DNA-transDDP antibodies with horse radish peroxidase or alkaline phosphotase conjugated antibodies to rabbit Ig and protein A-peroxidase - for hybrids visualization. This method allows to detect 2 pg/mm DNA.

Published
1991

2. [Multiple hybridization in genome analysis. 1. Reaction of diamine and bisulfite with cytosine for the introduction of nonradioactive markers into DNA].

Author

Turchinskiĭ MF, Aĭnbinder EI, and Sverdlov ED

Subjects
Animals, Bacteriophage lambda metabolism, Biotin, Cattle, Chemical Phenomena, Chemistry, DNA Transposable Elements, DNA, Viral metabolism, Diamines, Fluorescein-5-isothiocyanate, Fluoresceins, Indicators and Reagents, Nucleic Acid Hybridization, Sulfites, Thiocyanates, Cytosine, Genes
Abstract

A two-step chemical method has been developed for the introduction of biotin and other low-molecular derivatives into DNA. The method is based on the interaction of aliphatic diamines with cytosine residues of the polynucleotide chain in the presence of sodium bisulfite with subsequent addition of biotin. DNA modified this way may be used as an effective probe for non-isotopic hybridization which can be used simultaneously with 32P-labelled probes.

Published
1988

3. [Contacts of ribosomal proteins with tRNAPhe and 16S RNA in analogs of the 30S initiation complex].

Author

Abdurashidova GG, Nargizian MG, Rudenko NV, Turchinskiĭ MF, and Budovskiĭ EI

Subjects
Bacterial Proteins genetics, Bacterial Proteins metabolism, Escherichia coli metabolism, Peptide Initiation Factors genetics, Poly U metabolism, RNA, Bacterial genetics, RNA, Transfer, Amino Acyl genetics, Ribosomal Proteins genetics, Ultraviolet Rays, Escherichia coli genetics, Peptide Initiation Factors metabolism, RNA, Bacterial metabolism, RNA, Transfer, Amino Acyl metabolism, Ribosomal Proteins metabolism
Abstract

Direct RNA-protein contacts have been studied by means of ultraviolet-induced (254 nm) cross-links inside complexes of NAcPhe-tRNAPhe, Phe-tRNAPhe and deacylated tRNAPhe with poly(U)-charged 30S subunit of Escherichia coli ribosome. In the first two complexes tRNA directly contacts with the similar sets of proteins (S4, S5, S7, S9/S11; S6 and S8 are found only in the second complex). These sets are similar to that in the fMet-tRNAfMet X 30S X mRNA complex, evidencing similar disposition of tRNAs in these three complexes. 16S RNA contacts in free 30S subunit mainly with proteins S4, S7 and S9/S11. In both complexes, containing NAcPhe-tRNAPhe and Phe-tRNAPhe, 16S RNA contacts with essentially the same proteins (S4, S5, S7, S8, S9/S11, S10, S15, S16 and S17) and in the same ratio, evidencing similar conformation of 30S subunit in these two complexes. In the third complex deacylated tRNAPhe contacts with proteins S4, S5, S6, S8, S9/S11 and S15, 16S RNA-protein interaction differs from those in the first two complexes by a remarkable decrease of cross-linked proteins S8, and S9/S11 and by the appearance of a large amount of cross-linked proteins(s) S13/S14. Hence, this complex differs from the first two by conformation of 30S subunit and, probably, by disposition and/or conformation of tRNA.

Published
1985

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