1. [Genetic modification of Methylobacterium extorquens G10 producer strain of polyhydroxybutyrate].
- Author
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Fedorov DN, Zamakhaeva SA, Ezhov VA, Doronina NV, and Trotsenko IuA
- Subjects
- Bacterial Proteins metabolism, Carotenoids deficiency, Culture Media chemistry, Gene Deletion, Gene Expression, Genetic Engineering, Kinetics, Ligases metabolism, Methylobacterium extorquens metabolism, Molecular Weight, Oxidoreductases deficiency, Oxidoreductases genetics, Plasmids, Polymerization, Bacterial Proteins genetics, Hydroxybutyrates metabolism, Ligases genetics, Methanol metabolism, Methylobacterium extorquens genetics, Polyesters metabolism
- Abstract
The effect of the increased copy number of polyhydroxybutyrate (PHB) biosynthesis genes in pink-pigmented methylobacterium Methylobacterium extorquens G10 on properties of the biopolymer was studied. The activity of poly-3-hydroxybutyril-synthase (PHB-synthase) was shown to increase and the molecular weight of synthesized PHB decreases twofold (150 --> 79 kDa) after insertion of extra copies of phaC and phaCAB genes into cells of the producer strain, whereas the physicochemical properties of the plastic changed insignificantly. White mutant M. extorquens G10-W with disrupted synthesis of the carotenoid pigment (defect by the crtI gene, which codes for phytoene desaturase) was established to have the same rate of growth and level of PHB accumulation as the initial strain G10. The G10-W strain is a promising producer of PHB, with decreased expenses for purification and PHB biosynthesis.
- Published
- 2014