Search

Potato extract (PE), corn extract (CE) and papaya extract (PAE) significantly enhanced plantlet regeneration from PLBs (protocorm-like bodies) when 50 mL L - 1 of an extract was supplemented to the New Phalaenopsis (NP) medium containing sucrose. Among the treatments, CE 50 mL L - 1 induced the highest rate (66.63%) of plantlet regeneration from PLBs. Organic extracts of potato, corn and papaya at 50-100 mL L - 1 in NP medium also enhanced the subsequent growth of mini-plantlets. However, a higher level (200 mL L - 1 ) of extract in the medium inhibited the growth of the plantlets. Among these extracts, PE at 100 mL L - 1 showed the optimum effect on the acceleration of shoot growth while CE at the same concentration was optimum for root growth.

Potato extract (PE), corn extract (CE) and papaya extract (PAE) at concentrations of 25, 50 and 100 mL L - 1 enhanced callus growth on New Phalaenopsis (NP) medium containing sucrose. Among the various concentrations, 100mL L - 1 PE or PAE and 200 mL L - 1 CE significantly promoted callus growth compared to the control. Regeneration of protocorm-like bodies (PLBs) from calli was optimum when NP medium (sometimes referred to as BM) was supplemented with 100 mL L - 1 CE, followed by PE and PAE at the same concentration. Most plantlets also regenerated from PLBs with one 100 mL L - 1 CE.