1. Diabetes mellitus- and cooling-induced bladder contraction: an in vitro study
- Author
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Serpil Kalkan, Hakkı Gökbel, Kısmet Esra Nurullahoğlu Atalık, Nilsel Okudan, Gokhan Cuce, and Selçuk Üniversitesi
- Subjects
Detrusor muscle ,medicine.medical_specialty ,Carbachol ,Contraction (grammar) ,Physiology ,Urinary system ,medicine.medical_treatment ,Urinary Bladder ,Intraperitoneal injection ,Isometric exercise ,In Vitro Techniques ,Streptozocin ,Diabetes Mellitus, Experimental ,Potassium Chloride ,Rats, Sprague-Dawley ,Diabetes mellitus ,Internal medicine ,medicine ,Animals ,Rat urinary bladder ,business.industry ,Urinary Bladder Diseases ,Muscle, Smooth ,General Medicine ,Streptozotocin ,medicine.disease ,Acetylcholine ,Rats ,Cold Temperature ,Endocrinology ,medicine.anatomical_structure ,Female ,Cooling ,business ,Injections, Intraperitoneal ,Muscle Contraction ,medicine.drug - Abstract
PubMed: 20859065, The effects of diabetes mellitus during cooling on ACh-and KCl-induced responses were investigated in rat urinary bladder. Diabetes was induced in the rats by 50 mg/kg streptozotocin via an intraperitoneal injection. Rats' body and bladder weights were measured. The isometric tension to ACh (10-9 -3 × 10-4 M) and KCl (5-100 mM) in strips of urinary detrusor muscle of diabetic and non-diabetic rats, in organ baths at 37 and 28°C were recorded. The body weights were significantly decreased and the bladder weights increased in STZ-induced diabetic group compared to the non-diabetic group. ACh and KCl caused concentration-dependent contractions of urinary bladders from non-diabetic and STZ-induced diabetic rats. During cooling, the sensitivity and the maximal response were significantly higher than those during 37°C, both in non-diabetic and diabetic preparations. Cooling of detrusor muscle preparations induces a graded contraction inversely proportional to the temperature in diabetic rats. It may be assumed that the cooling response involves the same mechanisms in the diabetic and non-diabetic animals.
- Published
- 2010
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