1. Impact of matrix properties on the survival of freeze-dried bacteria.
- Author
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Wessman P, Mahlin D, Akhtar S, Rubino S, Leifer K, Kessler V, and Håkansson S
- Subjects
- Arthrobacter cytology, Arthrobacter isolation & purification, Calorimetry, Differential Scanning, Cellulose analogs & derivatives, Cellulose chemistry, Cellulose pharmacology, Cellulose ultrastructure, Colony Count, Microbial, Ficoll chemistry, Ficoll pharmacology, Ficoll ultrastructure, Freeze Drying, Hydrophobic and Hydrophilic Interactions, Hypromellose Derivatives, Methylcellulose analogs & derivatives, Methylcellulose chemistry, Methylcellulose pharmacology, Microscopy, Electron, Scanning, Polyvinyl Alcohol chemistry, Polyvinyl Alcohol pharmacology, Pseudomonas putida cytology, Pseudomonas putida isolation & purification, Sphingomonas cytology, Sphingomonas isolation & purification, Structure-Activity Relationship, Sucrose chemistry, Sucrose pharmacology, Surface Tension, Surface-Active Agents chemistry, Surface-Active Agents pharmacology, Transition Temperature, X-Ray Diffraction, Arthrobacter drug effects, Cryoprotective Agents chemistry, Cryoprotective Agents pharmacology, Microbial Viability drug effects, Pseudomonas putida drug effects, Sphingomonas drug effects
- Abstract
Background: Disaccharides are, in general, the first choice as formulation compounds when freeze-drying microorganisms. Although polysaccharides and other biopolymers are considered too large to stabilise and interact with cell components in the same beneficial way as disaccharides, polymers have been reported to support cell survival. In the present study we compare the efficiency of sucrose and the polymers Ficoll, hydroxyethylcellulose, hydroxypropylmethylcellulose and polyvinylalcohol to support the survival of three bacterial strains during freeze drying. The initial osmotic conditions were adjusted to be similar for all formulations. Formulation characterisation was used to interpret the impact that different compound properties had on cell survival., Results: Despite differences in molecular size, both sucrose and the sucrose-based polymer Ficoll supported cell survival after freeze drying equally well. All formulations became amorphous upon dehydration. Scanning electron microscopy and X-ray diffraction data showed that the discerned differences in structure of the dry formulations had little impact on the survival rates. The capability of the polymers to support cell survival correlated with the surface activity of the polymers in a similar way for all investigated bacterial strains., Conclusion: Polymer-based formulations can support cell survival as effectively as disaccharides if formulation properties of importance for maintaining cell viability are identified and controlled., (Copyright © 2011 Society of Chemical Industry.)
- Published
- 2011
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