1. Phage antibodies against an unstable hapten: oxygen sensitive reduced flavin.
- Author
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Bruggeman YE, Boogert A, van Hoek A, Jones PT, Winter G, Schots A, and Hilhorst R
- Subjects
- Amino Acid Sequence, Antibodies, Viral immunology, Molecular Sequence Data, Oxidation-Reduction, Oxygen, Bacteriophage M13 immunology, Flavins immunology, Haptens immunology, Immunoglobulin Fragments immunology
- Abstract
It is difficult to raise antibodies against haptens and antigens that are unstable under the physiological conditions of the serum. Here we have used a phage antibody library to isolate antibody fragments against oxygen sensitive reduced flavin, by selection of the phage under anaerobic and reducing conditions at pH 5 and a pre-elution step with the oxidized flavin. The binding of the reduced hapten to one of the antibody fragments was characterised by time-resolved polarised fluorescence, and shown to be highly specific for the reduced flavin.
- Published
- 1996
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