1. Overexpression of cyclin-dependent kinase 1 in esophageal squamous cell carcinoma and its clinical significance.
- Author
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Zhang HJ, Chen G, Chen SW, Fu ZW, Zhou HF, Feng ZB, Mo JX, Li CB, and Liu J
- Subjects
- Biomarkers, Tumor genetics, CDC2 Protein Kinase metabolism, Cell Proliferation genetics, China, Computational Biology methods, Esophageal Neoplasms genetics, Esophageal Neoplasms metabolism, Esophageal Squamous Cell Carcinoma metabolism, Gene Expression genetics, Gene Expression Profiling methods, Gene Expression Regulation, Neoplastic genetics, Gene Regulatory Networks genetics, Humans, MicroRNAs genetics, Protein Interaction Maps genetics, RNA, Long Noncoding genetics, RNA, Messenger genetics, RNA-Seq, Transcriptome genetics, CDC2 Protein Kinase genetics, Esophageal Squamous Cell Carcinoma genetics
- Abstract
Cyclin-dependent kinase 1 (CDK1) plays a significant role in certain malignancies. However, it remains unclear whether CDK1 plays a role in esophageal squamous cell carcinoma (ESCC). The aim of this study was to analyze the expression and clinical value of CDK1 in ESCC. CDK1 protein in 151 ESCC tissues and 138 normal esophageal tissues was detected by immunohistochemistry. RNA-seq of eight pairs of ESCC and adjacent esophageal specimens was performed to evaluate the levels of CDK1 mRNA. Microarray and external RNA-seq data from 664 cases of ESCC and 1733 cases of control tissues were used to verify the difference in CDK1 expression between the two groups. A comprehensive analysis of all data was performed to evaluate the difference in CDK1 between ESCC tissues and control tissues. Further, functional enrichment analyses were performed based on differentially expressed genes (DEGs) of ESCC and co-expressed genes (CEGs) of CDK1. In addition, a lncRNA-miRNA-CDK1 network was constructed. The expression of CDK1 protein was obviously increased in ESCC tissues (3.540 ± 2.923 vs. 1.040 ± 1.632, P < 0.001). RNA-seq indicated that the mRNA level of CDK1 was also highly expressed in ESCC tissues (5.261 ± 0.703 vs. 2.229 ± 1.161, P < 0.0001). Comprehensive analysis revealed consistent up-regulation of CDK1 (SMD = 1.41; 95% CI 1.00-1.83). Further, functional enrichment analyses revealed that the functions of these genes were mainly concentrated in the cell cycle. A triple regulatory network of PVT1-hsa-miR-145-5p/hsa-miR-30c-5p-CDK1 was constructed using in silico analysis. In summary, overexpression of CDK1 is closely related to ESCC tumorigenesis., (© 2021 The Authors. FEBS Open Bio published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies.)
- Published
- 2021
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