1. A translational enhancer element on the 3'-proximal end of the Panicum mosaic virus genome.
- Author
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Batten JS, Desvoyes B, Yamamura Y, and Scholthof KB
- Subjects
- 3' Untranslated Regions chemistry, 3' Untranslated Regions genetics, Base Sequence, Molecular Sequence Data, Mutation genetics, Nucleic Acid Conformation, Tombusviridae chemistry, Genome, Viral genetics, Protein Biosynthesis genetics, Regulatory Sequences, Ribonucleic Acid genetics, Tombusviridae genetics
- Abstract
Panicum mosaic virus (PMV) is a single-stranded positive-sense RNA virus in the family Tombusviridae. PMV genomic RNA (gRNA) and subgenomic RNA (sgRNA) are not capped or polyadenylated. We have determined that PMV uses a cap-independent mechanism of translation. A 116-nucleotide translational enhancer (TE) region on the 3'-untranslated region of both the gRNA and sgRNA has been identified. The TE is required for efficient translation of viral proteins in vitro. For mutants with a compromised TE, addition of cap analog, or transposition of the cis-active TE to another location, both restored translational competence of the 5'-proximal sgRNA genes in vitro.
- Published
- 2006
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