1. Small extravesicular microRNA in head and neck squamous cell carcinoma and its potential as a liquid biopsy for early detection.
- Author
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Galiveti CR, Kuhnell D, Biesiada J, Zhang X, Kelsey KT, Takiar V, Tang AL, Wise-Draper TM, Medvedovic M, Kasper S, and Langevin SM
- Subjects
- Humans, Squamous Cell Carcinoma of Head and Neck genetics, Case-Control Studies, Liquid Biopsy, Papillomaviridae genetics, MicroRNAs genetics, Papillomavirus Infections genetics, Head and Neck Neoplasms diagnosis, Head and Neck Neoplasms genetics
- Abstract
Background: The objective was to assess secretion of small extracellular vesicular microRNA (exo-miRNA) in head and neck squamous cell carcinoma (HNSCC) according to human papillomavirus (HPV) status, and determine the translational potential as a liquid biopsy for early detection., Methods: This study employed a combination of cell culture and case-control study design using archival pretreatment serum. Small extracellular vesicles (sEV) were isolated from conditioned culture media and human serum samples via differential ultracentrifugation. miRNA-sequencing was performed on each sEV isolate., Results: There were clear exo-miRNA profiles that distinguished HNSCC cell lines from nonpathologic oral epithelial control cells. While there was some overlap among profiles across all samples, there were apparent differences in exo-miRNA profiles according to HPV-status. Importantly, differential exo-miRNA profiles were also apparent in serum from early-stage HNSCC cases relative to cancer-free controls., Conclusions: Our findings indicate that exo-miRNA are highly dysregulated in HNSCC and support the potential of exo-miRNA as biomarkers for HNSCC., (© 2022 The Authors. Head & Neck published by Wiley Periodicals LLC.)
- Published
- 2023
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