1. Deregulation of selected microRNAs in sinonasal carcinoma: Value of miR-21 as prognostic biomarker in sinonasal squamous cell carcinoma.
- Author
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Kovarikova H, Bubancova I, Laco J, Sieglova K, Vosmikova H, Vosmik M, Dundr P, Nemejcova K, Michalek J, Palicka V, and Chmelarova M
- Subjects
- Aged, Analysis of Variance, Biomarkers, Tumor genetics, Biopsy, Needle, Carcinoma, Squamous Cell genetics, Carcinoma, Squamous Cell mortality, Carcinoma, Squamous Cell surgery, Case-Control Studies, Female, Gene Expression Profiling, Humans, Immunohistochemistry, Kaplan-Meier Estimate, Male, Middle Aged, Paranasal Sinus Neoplasms mortality, Paranasal Sinus Neoplasms surgery, Prognosis, Real-Time Polymerase Chain Reaction methods, Reproducibility of Results, Statistics, Nonparametric, Survival Analysis, Up-Regulation, Carcinoma, Squamous Cell pathology, Gene Expression Regulation, Neoplastic, MicroRNAs genetics, Paranasal Sinus Neoplasms genetics, Paranasal Sinus Neoplasms pathology
- Abstract
Background: Tumors occurring in the sinonasal area are characterized by unfavorable outcome due to difficult diagnosis, treatment, and prognosis of the disease corresponding with the anatomic complexity of the area., Methods: We used quantitative real-time polymerase chain reaction (PCR) to compare relative expression of miR-21, miR-141, and miR-200c in 70 formalin-fixed, paraffin-embedded samples of sinonasal carcinoma tissue (majority of squamous cell carcinoma [SCC] samples) with 17 control samples of sinonasal tissue., Results: Our data showed significant upregulation of miR-21 in sinonasal cancer tissue. Expression levels of miR-141 and miR-200c were below detectable levels in both sinonasal cancer samples and healthy tissue. Kaplan-Meier analysis with log-rank survival showed that patients with SCC with high expression of miR-21 (highest quartile) had impaired survival close to reaching statistical significance (Pā=ā.0630)., Conclusion: Our results suggest that miR-21 upregulation is involved in tumorigenesis of sinonasal carcinoma and that it is associated with poor prognosis. Thus, miR-21 could be used as a valuable prognostic biomarker., (© 2017 Wiley Periodicals, Inc.)
- Published
- 2017
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