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To investigate the potential targets and related mechanisms of micheliolide (MCL) in alleviating gouty arthritis (GA). The network pharmacology technology was used to retrieve MCL and GA related targets, screen their intersection targets, construct protein interaction maps and screen core targets. Enrichment analysis of Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) was conducted, the ″MCL-target-pathway-GA″ network diagram was drawn, and molecular docking verification of MCL and core target was conducted. Results A total of 778 MCL related targets, 351 GA related targets and 58 intersection targets were screened out. Twenty-four targets were predicted to be the core targets of MCL inalleviating GA, such as TNF, IL-1B (IL-1β) and so on. GO analysis confirmed 294 items related to biological process (BP), 38 items related to cellular components (CC) and 57 items related to molecular function (MF). KEGG analysis revealed 105 signaling pathways, including inflammation, metabolism, infection and tumor. The molecular docking results showed that MCL binds tightly to the core target of TNF and IL-1B (IL-1β) by hydrogen bonding, and the MCL binds most tightly to the core target of IL-1B (IL-1β). The core targets and related biological processes and signaling pathways of MCL alleviating GA were preliminarily predicted, and molecular docking verification was carried out, providing ideas for further in vivo and in vitro experiments. [ABSTRACT FROM AUTHOR]

In this paper, the effect of streptozotocin (STZ) on gonadal function in diabetic mice was studied. Seventy male Kunming mice were randomly divided into control group (10 rats) and model group (60 rats). Model group was intraperitoneally injected with STZ (75 mg./kg) for 2 days, 1 time/d,and the control group. The citrate buffer was injected intraperitoneally. Fasting blood glucose (FBG) was measured 72 hours after the last injection, and the mice in the model group with blood glucose≥11. 1 mmol/L were taken for follow-up test. Fasting blood glucose was measured at 7 d, 14 d,21 d and 28 d,and the mating experiment was carried out. The number of straddles and the number of matings were sacrificed after the 28 d mating experiment, and serum testosterone levels were determined. The results showed that compared with the control group,the blood glucose of the model group was sig-nificantly increased at 7 d,14 d.21 d and 28 d after STZ injection (P<0.05); and the number of straddles in the mating test at 7 d, 14 d,21 d and 28 d and The number of matings was significantly reduced (P<0.05,P<70.01), and serum testosterone levels were significantly decreased in 28 d mice (P<0. 01). Therefore,STZ low-dose multiple intraperitoneal injection can successfully induce hypogonadism in diabetic mice. [ABSTRACT FROM AUTHOR]