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RNA has been isolated from adult Nip- postrongylus brasiliensis (NB) homogenized in guani- dium isothiocyanate. The mRNA has been translated in vitro utilizing a rabbit reticulocyte lysate system and translated products immunoprecipitated with rabbit anti-adult NB antiserum and rat infection serum. ABSTRACT: RNA has been isolated from adult Nip- postrongylus brasiliensis (NB) homogenized in guani- dium isothiocyanate. The mRNA has been translated in vitro utilizing a rabbit reticulocyte lysate system and translated products immunoprecipitated with rabbit anti-adult NB antiserum and rat infection serum.

Although eosinophils and other inflammatory cells from the circulation and peritoneal cavity can damage Trichinella spiralis newborn larvae (NBL) in vitro, the cytotoxic potential of cells from the intestinal lamina propria, a site that may be the first line of defense against NBL migration, is unknown. Accordingly, we examined the interaction between NBL and isolated intestinal lamina propria cells (ILPC), including an enriched eosinophil population, from rats and humans. Rat ILPC killed NBL in vitro only after a prolonged incubation of 6 days. However they strongly adhered to NBL after only 4 hr incubation and prevented muscle establishment of NBL injected intravenously. Human ILPC showed similar adherence as rat ILPC but no killing was seen at the incubation time tested (36 hr).

On irradiation of 2-phenylethyl-l-C/sup 14/ iodide with Co/sup 60/ gamma -rays, 2-phenylethyl-2-C/sup 14/ iodide was formed with a G value oi 0.50 plus or minus 0.05 for the dosage range of 8.8 to 47.9 million rads. The mechanism for this isotope position rearrangement likely involves a 1,2-phenyl shift of the 2e G values for total decomposition of 2-phenylethyl iodide and for ethylbenzene formation were about 2 and 0.4, respectively. Radiolysis of ordinary 2-phenylethyl iodide showed that G/sub 1/2I2/ was 2.0 plus or minus 0.1 for the dosage range of 7.3 to 22.4 million rads. Another experiment with ordinary 2-phenylethyl iodide irradiated with 38 million rads showed G/sub HI/ to be 0.2 and that the organic products of radiolysis, measured by vapor phase chromatography, were styrene, ethylbenzene, and toluene in relative amounts of 82: 17: 1, respectively. (auth)