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The viral diseases that occurred in recent years have increased the interest in non-toxic to healthy cells and naturally isolated agents to struggle with these diseases. The key intention of this research is to examine both antiviral potentials against the Infectious Bronchitis model virus (IBV) and cytotoxic activities on determined cell lines of different active ingredients and medical herbs extracts for developing new antiviral agents or drugs towards SARS-CoV-2. The antiviral potency of the samples against IBV was determined as in ovo virucidal antiviral activity in specific pathogen-free (SPF) embryonated chicken eggs (ECEs). To detect antiviral activity, the haemagglutination test was performed after 48 h of incubation for all samples. The cytotoxic activity of the samples was identified on HepG2, Caco-2, HeLa, HEK293, PANC-1, PC-3, A549, MDAMB- 231, and CCD-34Lu cell lines by the MTT protocol. Hypericum perforatum extract was found to have a dominant role in cytotoxicity and antiviral activity. In addition, while nobiletin and Sambucus nigra do not exhibit cytotoxic activity on cells, they play a significant role in antiviral activity. As a consequence of our investigation, the cytotoxic and antiviral properties of Laurus nobilis, H. perforatum, and S. nigra extracts were found remarkable and the potential of these extracts was demonstrated.