1. Alterations in Toll-Like Receptor 7 and -9 mRNA Levels in Lungs after Ovariohysterectomy in a Pyometra Mouse Model.
- Author
-
Kyriakopoulos K, Katsimpoulas M, Mylonas KS, Lidoriki I, Ziogas IA, Perivolioti EP, Stamataki DK, Chrelias C, Schizas D, Alexandrou A, Liakakos T, and Kapelouzou A
- Subjects
- Animals, Disease Models, Animal, Female, Humans, Lung metabolism, Mice, Mice, Inbred C57BL, Myeloid Differentiation Factor 88 genetics, Myeloid Differentiation Factor 88 metabolism, NF-kappa B genetics, NF-kappa B metabolism, RNA, Messenger, Toll-Like Receptor 7 metabolism, Toll-Like Receptor 9 genetics, Toll-Like Receptor 9 metabolism, Lung Injury metabolism, Lung Injury pathology, Pyometra metabolism, Pyometra pathology
- Abstract
Background: Pyometra (P) leads to sepsis and multiple organ dysfunction syndrome. Toll-like receptors (TLRs) recognize pathogens which can cause P. The aim of this study was to investigate TLR-7 and -9 via the MYD88 pathway and the nuclear factor kappa B (NFκB) response in the uterus of a P mouse model before and after ovariohysterectomy (RP) as well as potential lung injury., Materials and Methods: 200 female C57BL/6J mice were randomly divided into groups (N = 10/subgroup; sham 1, 2, 3, 7; P1, 2, 3, 7; 1RP1, 2, 3, 7; 2RP1, 2, 3, 7; 3RP1, 2, 3, 7) according to the day of euthanasia. Pathogens were administrated in the groups P and RP in order to induce P., Results: Alterations in blood chemistry, histopathology, and RT-qPCT analysis before (P) and after RP were observed. Significant correlations were also found between MYD88, NFκB, and TLR9 in P and RP groups in the lungs and in RP groups in the uterus, suggesting that the immune system responded via the TLR9-MYD88 pathway., Conclusions: This is the first report of immunohistochemical TLR-7 and -9 localization and of TLR-7, -9, MYD88, and NFκB mRNA expression in the uterus causing lung injury in a P mouse model., (© 2021 S. Karger AG, Basel.)
- Published
- 2022
- Full Text
- View/download PDF