1. Biocompatibility of PLGA/sP(EO-stat-PO)-coated mesh surfaces under constant shearing stress.
- Author
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Böhm G, Ushakova Y, Alizai HP, Braunschweig T, Lente C, Heffels KH, Groll J, Neumann UP, and Junge K
- Subjects
- Animals, Apoptosis, Biocompatible Materials, Cell Proliferation, Coated Materials, Biocompatible adverse effects, Collagen metabolism, Disease Models, Animal, Female, Hernia, Diaphragmatic metabolism, Hernia, Diaphragmatic pathology, Hernia, Diaphragmatic surgery, Herniorrhaphy adverse effects, Herniorrhaphy methods, Macrophages pathology, Materials Testing, Microscopy, Electron, Scanning, Polyethylene Glycols adverse effects, Polyglactin 910 adverse effects, Rabbits, Shear Strength, Stress, Mechanical, Tissue Adhesions etiology, Tissue Adhesions pathology, Tissue Scaffolds adverse effects, Tissue Scaffolds chemistry, Coated Materials, Biocompatible chemistry, Polyethylene Glycols chemistry, Polyglactin 910 chemistry, Surgical Mesh adverse effects
- Abstract
Background: In order to allow inflammatory response modification and ultimately improvement in tissue remodeling, we developed a new surface modification for meshes that will serve as a carrier for other substances. Biocompatibility is tested in an animal model., Methods: The animal model for diaphragmatic hernia repair was established in prior studies. Meshes were surface modified with star-configured PEO (polyethylene oxide)-based molecules [sP(EO-stat-PO)]. An electrospun nanoweb of short-term absorbable PLGA (polylactide-co-glycolide) with integrated sP(EO-stat-PO) molecules was applied onto the modified meshes. This coating also served as aerial sealing of the diaphragm. A final layer of hydrogel was applied to the product. Adhesive properties, defect size and mesh shrinkage were determined, and histological and immunohistochemical investigations performed after 4 months., Results: The mean defect size decreased markedly in both modified mesh groups. Histologically and with regard to apoptosis and proliferation rate, smooth muscle cells, collagen I/III ratio and macrophage count, no statistically significant difference was seen between the 3 mesh groups., Conclusions: In this proof-of-principle investigation, we demonstrate good biocompatibility for this surface-modified mesh compared to a standard polypropylene-based mesh. This new coating represents a promising tool as a carrier for bioactive substances in the near future., (Copyright © 2011 S. Karger AG, Basel.)
- Published
- 2011
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