1. Aspirin Increases Apolipoprotein-A-I-Mediated Cholesterol Efflux via Enhancing Expression of ATP-Binding Cassette Transporter A1.
- Author
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Wang, Yu-Hua, Chen, Yan-Fang, Chen, Shao-Rui, Chen, Xi, Chen, Jian-Wen, Shen, Xiao-Yan, Mou, Yong-gao, and Liu, Pei-Qing
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ASPIRIN , *APOLIPOPROTEINS , *CHOLESTEROL , *GENE expression , *PROTEIN binding , *ADENOSINE triphosphate , *REVERSE transcriptase polymerase chain reaction , *DRUG dosage , *PEROXISOMES , *ATHEROSCLEROSIS - Abstract
Background: The efflux of cellular cholesterol mediated by apolipoprotein (apo)A-I and ATP-binding cassette transporter A1 (ABCA1) is a major pathway of reverse cholesterol transport. We investigated the effect of aspirin on this process. Methods: The expression levels of ABCA1 in RAW264.7 cells were determined using reverse transcription polymerase chain reaction and Western blot analysis. 3H-cholesterol efflux was measured by scintillation counting. Results: 0.5 mmol/l aspirin increased apoA-I-mediated cholesterol efflux and increased the expression of ABCA1. By increasing the dose of aspirin higher than 0.5 mmol/l, ABCA1 expression and function were significantly decreased. In cells transfected with a specific peroxisome proliferator-activated receptor (PPAR)-α small interfering RNA, the induction of ABCA1 expression and apoA-I-mediated 3H-cholesterol efflux by aspirin were substantially suppressed. Conclusions: The data demonstrate that low-dose aspirin increases ABCA1 expression via a PPAR-α-dependent mechanism and increases apoA-I-mediated cholesterol efflux. Copyright © 2010 S. Karger AG, Basel [ABSTRACT FROM AUTHOR]
- Published
- 2010
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