1. The Immunoprotective Role of Interleukin-10 in Abnormal Pregnancy Outcome Induced by Toxoplasma gondii Infection.
- Author
-
Zhang, Ruijin, Zhang, Haixia, Liu, Xianbing, Fu, Qiang, Xu, Xiaoyan, and Hu, Xuemei
- Subjects
- *
IMMUNOLOGY , *INTERLEUKIN-10 , *INTERLEUKINS , *TOXOPLASMA gondii , *PREGNANCY - Abstract
Aims: Toinvestigate the immunoprotective effect of IL-10 on pregnancy in Toxoplasma gondii-infected C57BL/6 mice. Methods: Twenty-four pregnant mice were randomly divided into 3 equal groups, i.e. a control group (CG), an infected group (IG) and an infected group treated with IL-10 (IPTG). Each IPTG and IG mouse was infected with 400 T. gondii trophozoites on gestational day (GD) 8. Each IPTG mouse was injected with 1 μg recombinant mouse IL-10 via a tail vein on GD 10 and 12. The mice were sacrificed on day 7 postinfection. Qa-1 and NKG2A were analyzed in the placenta by flow cytometry and real-time PCR. IL-4, IL-10 and IFN-γ in the placenta supernatant were analyzed by ELISA. Statistical analysis was performed by one-way ANOVA. Results: IPTG mice showed a better mental state, had larger fetuses and placentas with a better blood supply and a lower resorption ratio compared to IG mice. NKG2A and Qa-1 were significantly increased in IPTG mice compared to IG mice (p < 0.05). IL-4 and IL-10 in IPTG placenta supernatant were increased (p < 0.05), but IFN-γ was decreased (p < 0.05) compared to IG placenta supernatant. The ratios of IFN-γ/IL-4 and IFN-γ/IL-10 were decreased in IPTG mice compared to IG mice (p < 0.05, p < 0.05). Conclusion: IL-10 plays an immunoprotective role and improves the pregnancy outcome of T. gondii-infected mice. Copyright © 2011 S. Karger AG, Basel [ABSTRACT FROM AUTHOR]
- Published
- 2012
- Full Text
- View/download PDF