1. Hydrogen sulfide serves as a biomarker in the anterior segment of patients with diabetic retinopathy.
- Author
-
Han Y, Zhang X, Zhou Z, Yu M, Wang Q, Yao J, and Han Y
- Subjects
- Aqueous Humor metabolism, Biomarkers metabolism, Enzyme-Linked Immunosorbent Assay, Female, Humans, Male, Middle Aged, Anterior Eye Segment metabolism, Diabetic Retinopathy metabolism, Hydrogen Sulfide metabolism
- Abstract
Objective: The present study aims to determine hydrogen sulfide (H
2 S) concentrations of the aqueous humor from patients with diabetic retinopathy (DR) to compare its levels in the anterior segments, also to investigate its effect on the retinal microvascular endothelial cells under high glucose condition., Methods: AH samples were collected from patients with proliferative diabetic retinopathy (n = 11), non-proliferative diabetic retinopathy (n = 12) and diabetic patients without DR as controls (n = 12). There were 5 patients with PDR received intraocular anti-VEGF injection (Lucentis). Cultured RF/6A cells were grouped into control group, mannitol group, high glucose group and NaHS co-administrated high glucose group. Concentrations of H2 S were detected by chemical assay. Cell apoptosis was evaluated by flow cytometry., Results: A significantly higher H2 S level was observed in AH samples of PDR patients among other groups. The H2 S level of DR group was higher than that of control group. Decreased H2 S levels in the AH of post-injected PDR patients were observed compared with their AH samples before the anti-VEGF injection. In cell culture, low concentration of NaHS can reverse high-glucose-induced apoptosis of RF/6A cells., Conclusion: Our study revealed increased H2 S levels in the anterior segments of different DR patients. The anti-VEGF injection reduced the H2 S level in AH from PDR patients. The study suggested that H2 S may serve as a biomarker in the progression of PDR. On the other hand, the H2 S donor exerted a protective effect on retinal vascular endothelial cells against high-glucose-induced apoptosis.- Published
- 2020
- Full Text
- View/download PDF