1. Platycodin D Attenuates Airway Inflammation in a Mouse Model of Allergic Asthma by Regulation NF-κB Pathway.
- Author
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Zhang T, Yang S, Du J, Jinfu Y, and Shumin W
- Subjects
- Animals, Asthma chemically induced, Bronchoalveolar Lavage Fluid chemistry, Campanulaceae metabolism, Dexamethasone therapeutic use, Disease Models, Animal, Eosinophilia drug therapy, Eosinophils immunology, Female, I-kappa B Kinase metabolism, I-kappa B Proteins metabolism, Interleukin-13 metabolism, Interleukin-4 metabolism, Interleukin-5 metabolism, Lung pathology, Mice, Mice, Inbred BALB C, NF-KappaB Inhibitor alpha, Ovalbumin, Plant Extracts therapeutic use, Random Allocation, Transcription Factor RelA metabolism, Airway Resistance drug effects, Anti-Asthmatic Agents therapeutic use, Anti-Inflammatory Agents therapeutic use, Asthma drug therapy, Saponins therapeutic use, Triterpenes therapeutic use
- Abstract
In this work, the anti-asthma potential of platycodin D (PLD) was studied by investigation of its effect to suppress airway inflammation, a murine model of asthma and the possible mechanisms. A total of 50 mice were randomly assigned to five experimental groups: control, ovalbumin (OVA), OVA+dexamethasone (2 mg/kg) and OVA+PLD (40, 80 mg/kg). Airway resistance (Raw) were measured; airway histological studies were evaluated by the hematoxylin and eosin (HE) staining; interleukin-4 (IL-4), interleukin-5(IL-5), and interleukin-13 in bronchoalveolar lavage fluid (BALF) were evaluated by enzyme-linked immunosorbent assay (ELISA); NF-κBp65, p-NF-κBp65, p-IKKα, IKKα, p-IKKβ, p-IкBα, and IкBα of airway were measured by Western blotting. Our study demonstrated that PLD inhibited OVA-induced increases in Raw and eosinophil count in airway; IL-4, IL-5, and IL-13 were recovered in BALF. Histological studies demonstrated that PLD substantially inhibited OVA-induced eosinophilia in airway tissue. Western blotting studies demonstrated that PLD substantially inhibited NF-κB pathway. These findings suggest that PLD may effectively ameliorate the progression of asthma and could be used as a therapy for patients with allergic asthma.
- Published
- 2015
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