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Purpose The purpose of this manuscript was to investigate the effect of high and low speed blood flow restriction versus non-blood flow restriction isokinetic exercise on isometric muscle strength, muscle fatigue, and muscle contraction displacement. Methods The subjects were consisted of ten males without non-specific muscular skeletal disease. They were classified into four training groups: ① high speed(300°/s) without blood flow restriction, ② high speed(300°/s) under blood flow restriction, ③ low speed(90°/s) without blood flow restriction, ④ low speed(90°/s) under blood flow restriction. Before and after the intervention, all of the subjects underwent measurements of isometric quadriceps strength, muscle fatigue, and muscle contraction displacement. Results In regard to isometric quadriceps strength before & after intervention, there was no significant difference between groups. For the lactic acid measurement before & after intervention, there was a signigicant difference between groups(p