1. Immunological measurement of aspartate/alanine aminotransferase in predicting liver fibrosis and inflammation
- Author
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Young Lim Ham, Gwang Ho Baik, Hyun Jeong Kim, Suk Pyo Shin, Dong Joon Kim, Sang Yeol Kim, Chang Seok Bang, Eui Yul Choi, Ki Tae Suk, and Young Joo Yang
- Subjects
medicine.medical_specialty ,hepatitis b, chronic ,alanine aminotransferase ,Liver fibrosis ,Portal venous pressure ,aspartate transferase ,Inflammation ,digestive system ,Gastroenterology ,03 medical and health sciences ,0302 clinical medicine ,Fibrosis ,Internal medicine ,medicine ,Alanine aminotransferase ,medicine.diagnostic_test ,business.industry ,Hepatitis B ,medicine.disease ,Liver biopsy ,Immunoassay ,Medicine ,030211 gastroenterology & hepatology ,enzyme-linked immunosorbent assay ,medicine.symptom ,business - Abstract
Background/Aims Enzymatic analysis of aspartate/alanine aminotransferase (AST/ALT) does not exactly represent the progression of liver fibrosis or inflammation. Immunoassay for AST (cytoplasmic [c] AST/mitochondrial [m] AST) and ALT (ALT1/ALT2) has been suggested as one alternatives for enzymatic analysis. The objective of this study was to evaluate the efficacy of immunoassay in predicting liver fibrosis and inflammation. Methods A total of 219 patients with chronic hepatitis B (CHB) who underwent hepatic venous pressure gradient (HVPG) and liver biopsy before antiviral therapy were recruited. Serum samples were prepared from blood during HVPG. Results of biochemical parameters including enzymatic AST/ALT and immunological assays of cAST, mAST, ALT1, and ALT2 through sandwich enzyme-linked immunosorbent assay (ELISA) immunoassay with fluorescence labeled monoclonal antibodies were compared with the results of METAVIR stage of live fibrosis and the Knodell grade of inflammation. Results METAVIR fibrosis stages were as follows: F0, six (3%); F1, 52 (24%); F2, 88 (40%); F3, 45 (20%); and F4, 28 patients (13%). Mean levels of AST and ALT were 121 ± 157 and 210 ± 279 IU/L, respectively. Mean HVPG score of all patients was 4.7 ± 2.5 mmHg. According to the stage of liver fibrosis, HVPG score (p < 0.001, r = 0.439) and ALT1 level (p < 0.001, r = 0.283) were significantly increased in all samples from patients with CHB. ALT (p < 0.001, r = 0.310), ALT1 (p < 0.001, r = 0.369), and AST (p < 0.001, r = 0.374) levels were positively correlated with Knodell grade of inflammation. Conclusions ALT1 measurement by utilizing sandwich ELISA immunoassay can be useful method for predicting inf lammation grade and fibrosis stage in patients with CHB.
- Published
- 2020