1. Evaluation and Assessment of Biosafety Carotenoid-biofortified Rice (PAC): Progeny Stability Study for Four Generations.
- Author
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Sung-Dug Oh, Soo-Yun Park, Soo-In Sohn, Jae-Kwang Kim, Jong-Sug Park, Soon Ki Park, Hyun Suk Cho, Byung-Ohg Ahn, and Kijong Lee
- Subjects
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BIOSAFETY , *CAROTENOIDS , *PROGENY tests (Botany) , *PLANT genetics , *BIOMARKERS - Abstract
The carotenoid-biofortified (PAC) rice was developed and confirmed to synthesis carotenoids (zeaxanthin, α-carotene and β-carotene) in rice endosperm. For biosafety assessment of PAC rice, the stability of the insert genes in carotenoidbiofortified rice at genomic and protein expression level through different generations (T3~T6) was studied. Southern blot analysis confirmed that the multi-generation stability of the genes : PAC gene (CrtI and Psy), promoters (35S and Glb), terminators (NOS and PinII), selectable marker (bar) and MAR gene. It was also confirmed that the backbone DNA containing antibacterial gene (aadA) was not present in PAC rice. Immuno-strip assay (PAT) showed that the bar gene was stably expressed through generations. HPLC analysis confirmed that carotenoids were consistently detected through T3~T6 generations. These results will be filed to biosafety assessment document of PAC rice. [ABSTRACT FROM AUTHOR]
- Published
- 2012
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