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Background: Diabetes was one of the most important metabolic and public health diseases all over the world. It was the most common endocrine disease caused by metabolic disorders. Type 2 diabetes is rapidly progressing in world. The incidence of diabetes is predicted to double in the following 20 years. Objectives: The current study was aims to evaluate the effect of standard nutrition and exercise on glucose and lipid levels in diabetic patients. Patients and Methods: It was an intervention study whose target population is consisting of 120 diabetic patients who had referred to PARSIAN Diabetes Research Center. Sampling size has been non-randomly selected. Mean and standard deviation are quantitative data applied in this study. T-test and independent sample T-test have been respectively utilized to compare changes within and between the groups. The amount of p-value has been calculated less than 0.05 which indicates statistical significance. Results: The achieved findings shows that glucose level has significantly decreased in experiment group using a pretest-posttest design (P < 0.05). Conclusions: Eight-week standard nutrition and regular exercise have significant effect on decrease of glucose level, insulin resistance and plasma lipoproteins. It is suggested to apply these programs in diabetes centers.