Search

Copyright of Journal of Kunming Medical University / Kunming Yike Daxue Xuebao is the property of Kunming Medical Journal and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective To optimize the extraction process for carnosic acid from leaves of Rosmarinus officinalis Linn. Methods With the extraction rate of carnosic acid as quantitative index, the best extraction solvent and extraction method were identified by single factor experiments. And then, the effects of extraction time, solid-liquid ratio and extraction times on extraction rate of carnosic acid were investigated by orthogonal tests. Results The optimal extraction technology was as follows: ethyl acetate extracting with material and solvent ratio of 1: 14, ultrasonic extraction for 4 times, wand 75 min each time. Under these conditions, the average extraction rate of carnosic acid was up to 2.479%. Conclusion The optimal extraction process for carnosic acid from R. officinalis was reproducible and stable. [ABSTRACT FROM AUTHOR]

Objective To investigate the antiviral effect of ethanol extract from Laggera Pterodonta against influenza A vims in vitro. Methods The cytopathic effect (CPE) assay were used to explore the antiviral effect of ethanol extract against influenza vims and the possible target in the model of the prevention and treatment. Results The 50% cytotoxic concentration of ethanol extract was 3.07 mg/mL. In the model of prevention and treatment, the ethanol extract could inhibit the infection of influenza A virusPR8 strain in MDCK cell, the 50% inhibitory concentrations were 0.31mg/mL and 0.73mg/mL, the selection indexes (Si) were 9.90 and 4.21, respectively. Conclusion The ethanol extract from Laggera Pterodonta can inhibit the influenza virus infection in vitro, and the effects may be better in the model of prevention. [ABSTRACT FROM AUTHOR]