1. Saliva Pooling Strategy for the Large-Scale Detection of SARS-CoV-2, Through Working-Groups Testing of Asymptomatic Subjects for Potential Applications in Different Workplaces.
- Author
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Girón-Pérez DA, Ruiz-Manzano RA, Benitez-Trinidad AB, Ventura-Ramón GH, Covantes-Rosales CE, Ojeda-Durán AJ, Mercado-Salgado U, Toledo-Ibarra GA, Díaz-Reséndiz KJ, and Girón-Pérez MI
- Subjects
- Asymptomatic Infections, COVID-19 prevention & control, COVID-19 virology, Contact Tracing, Humans, RNA, Viral isolation & purification, SARS-CoV-2 genetics, Sensitivity and Specificity, Specimen Handling, Workplace, COVID-19 diagnosis, COVID-19 Nucleic Acid Testing methods, COVID-19 Testing methods, SARS-CoV-2 isolation & purification, Saliva virology
- Abstract
Objective: To perform an improved large-scale SARS-CoV-2 detection on pooled tests of asymptomatic workers., Methods: qRT-PCR validation of the SARS-CoV-2 detection in salivae samples and saliva pools and working-group saliva pooling and testing for SARS-CoV-2., Results: We found a high Cycle threshold correlation (r = 0.9099) between swabs and saliva samples. Then, through the pooling strategy, we detected that 18/360 (5%) of individual saliva samples were SARS-CoV-2 positive. Saliva-pooling efficiency (360 of test sample/30 individual PCR) was higher (5.45) than the reported for swabbing group-testing and we spared 82% of the PCR reagents as well as sampling and personal protection equipment., Conclusion: Through this simplified and less expensive procedure, we detected in a short time asymptomatic-infected SARS-CoV-2-carriers that were isolated from their co-workers, thus, this methodology can be implemented in different workplaces to ensure consumers that employees are not infectious., Competing Interests: MI Giroń-Pérez, DA Giroń-Pérez, Ruiz-Manzano, Benitez-Trinidad, Ventura-Ramoń, Covantes-Rosales, Ojeda-Durań, Mercado-Salgado, Toledo-Ibarra, and Díaz-Reséndiz have no relationships/conditions/circumstances that present potential conflict of interest., (Copyright © 2021 American College of Occupational and Environmental Medicine.)
- Published
- 2021
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