1. Immunohistochemical visualization of a metabotropic glutamate receptor.
- Author
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Görcs TJ, Penke B, Böti Z, Katarova Z, and Hámori J
- Subjects
- Amino Acid Sequence, Animals, Blotting, Western, Cerebellar Cortex anatomy & histology, Cerebellar Cortex immunology, Dendrites immunology, Dendrites metabolism, Epitopes, Hemocyanins immunology, Immunohistochemistry, Male, Microscopy, Electron, Molecular Sequence Data, Purkinje Cells immunology, Purkinje Cells metabolism, Rats, Rats, Wistar, Receptors, Glutamate immunology, Synaptic Membranes immunology, Synaptic Membranes metabolism, Synaptosomes immunology, Synaptosomes metabolism, Cerebellar Cortex metabolism, Receptors, Glutamate metabolism
- Abstract
The immunocytochemical localization of the recently cloned metabotropic glutamate receptor 1 alpha (mGluR1 alpha) was demonstrated with a C-terminus specific antibody in rat cerebellar cortex. This antibody detects a 138-140 kDa major, and a 46 kDa minor band in membrane preparations of rat cortex and cerebellum. mGluR1 alpha immunoreactivity (mGRi) was present in Purkinje and basket cells. Purkinje cell dendritic spines and their postsynaptic membranes showed selective labelling. Presynaptic membranes, parallel fibres and glial processes were devoid of mGRi. It is suggested that the selective postsynaptic localization of this receptor at the dendritic spines of Purkinje cells serves as the morphological basis for long term depression processes in the molecular layer of the cerebellar cortex.
- Published
- 1993
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