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Background: The use of hyaluronidase in hyaluronic acid vascular occlusion has been evaluated; however, the models used do not accurately assimilate the facial morphologic characteristics or study the effects on adjacent tissues. The purpose of this study was to determine an effective concentration of subcutaneous hyaluronidase to dissolve a hyaluronic acid embolism and its effect on surrounding tissue., Methods: Fifteen rabbits were divided into six groups. An inguinal incision was performed on the femoral artery to create a hyaluronic acid embolism in the control and treatment groups (low-, medium-, and high-hyaluronidase groups). Hyaluronidase was injected subcutaneously. Photographic follow-up, histologic analysis, and quantification of hyaluronic acid were performed. Kruskal-Wallis test and post hoc with Bonferroni correction (p < 0.05) was used to compare the presence of hyaluronic acid in the arterial lumen between groups., Results: Despite the persistence of intravascular hyaluronic acid, macroscopic and microscopic differences were found between the embolism control group and embolism hyaluronidase high-dose group. Histologic analysis demonstrated thrombosis throughout groups. Skeletal muscle was least affected in the embolism hyaluronidase 500 IU group with less lysis and inflammatory infiltrate., Conclusions: A 500 IU hyaluronidase dose partially prevents the damage caused by the embolism, and does not affect the surrounding tissue. The use of thrombolytic therapy combined with higher doses of hyaluronidase subcutaneously in this model is proposed., Competing Interests: Disclosure:The authors have no financial interests and no conflicts of interest to declare. The study was not supported by any funding source., (Copyright © 2021 by the American Society of Plastic Surgeons.)

There is an increasing demand of small-diameter vascular grafts for treatment of circulatory pathologies. Decellularization offers the possibility of using human blood vessels as scaffolds to create vascular grafts. Umbilical vessels have great potential because of their availability and morphological characteristics. Various decellularization techniques have been used in umbilical vessels, but consensus on which is the most appropriate has not yet been reached. The objective of this review is to analyze the morphological and biomechanical characteristics of decellularized human umbilical arteries and veins with different techniques. Evidence indicates that the umbilical vessels are a viable option to develop small-diameter vascular grafts. Detergents are the agents most often used and with most evidence. However, further studies are needed to accurately analyze the components of the extracellular matrix and biomechanical characteristics, as well as the capacity for recellularization and in vivo functionality.