1. Peripheral nerve regeneration through a long detergent-denatured muscle autografts in rabbits.
- Author
-
Mligiliche N, Tabata Y, Endoh K, and Ide C
- Subjects
- Animals, Electromyography, Electrophysiology, Microscopy, Electron, Motor Neurons physiology, Muscle Spindles ultrastructure, Muscle, Skeletal ultrastructure, Nerve Fibers, Myelinated ultrastructure, Peripheral Nerves pathology, Rabbits, Sciatic Nerve pathology, Sciatic Nerve physiopathology, Sciatic Nerve surgery, Tibial Nerve pathology, Tibial Nerve physiopathology, Tibial Nerve surgery, Transplantation, Autologous, Detergents pharmacology, Muscle, Skeletal drug effects, Muscle, Skeletal transplantation, Nerve Regeneration physiology, Peripheral Nerves physiopathology, Peripheral Nerves surgery, Sodium Dodecyl Sulfate pharmacology
- Abstract
Muscle segments excised from rabbit biceps femoris muscles were treated with detergent sodium dodecyl sulphate to denature cellular constituents, and each was autografted in a 5 cm gap of the sciatic nerve in the same rabbit. Axonal regrowth through the grafts and reinnervation into the host sciatic nerves and muscles were studied morphologically, and electrophysiologically, 4 months after grafting. Regenerating axons accompanied by Schwann cells extended through basal lamina tubes of the grafts into the distal host nerves. Reinnervation of the tibialis anterior muscles by motor nerves was confirmed by recovery of the compound muscle action potentials (CMAP) and the reinnervation of the muscle spindles was demonstrated by electron microscopy. These findings indicated that the basal lamina tubes of denatured muscles were effective scaffolds through which the regenerating nerve fibers grew across as large a gap as 5 cm.
- Published
- 2001
- Full Text
- View/download PDF