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[EN] The morphology of the larva, pupa, male, and female of Rhyacophila loeffleri Valladolid & Waringer, n. sp., from Austria, Czech Republic, France, and Italy is described. Data from molecular analysis are also provided, based on mitochondrial cytochrome c oxidase subunit I (mtCOI), including sequences from German and Swiss specimens, and compared with those of samples of the nominate species R. fasciata Hagen 1859, as well as with other species and subspecies in the Rhyacophila fasciata Species Complex: R. septentrionis McLachlan 1865, R. denticulata McLachlan 1879, R. sociata Navás 1916, R. kykladica Malicky & Sipahiler 1993, R. delici Kučinić & Valladolid 2020, R. fasciata viteceki Valladolid & Kučinić 2020 and R. macedonica Karaouzas, Valladolid & Ibrahimi 2022. Our study revealed morphological differences between R. fasciata and R. loeffleri, as well as genetic differences among the other taxa of the R. fasciata Complex, supporting the recognition of R. loeffleri as a new species. Also, Rhyacophila fasciata viteceki is recognized as a distinct species, R. viteceki (status promotus), based on results of the molecular analysis., [ES] Se ha estudiado la morfología de la larva, pupa, macho y hembra de Rhyacophila loeffleri Valladolid & Waringer (n. sp.) de Austria, República Checa, Francia e Italia. Se incluyen datos del análisis molecular del gen mitocondrial citocromo oxidasa subunidad I (COImt), incluidas secuencias de especímenes alemanes y suizos, que se compararon con muestras de la especie nominal R. fasciata Hagen 1859, así como con otras muestras de especies y subespecies del Complejo Rhyacophila fasciata: R. septentrionis McLachlan, 1865, R. denticulata McLachlan 1879, R. sociata Navás 1916, R kykladica Malicky & Sipahiler 1993, R. delici Kučinić & Valladolid 2020, R. fasciata viteceki Valladolid & Kučinić 2020 y R. macedonica Karaouzas, Valladolid & Ibrahimi 2022. Nuestro estudio reveló diferencias morfológicas entre R. fasciata y R. loeffleri, así como diferencias genéticas entre los otros taxones del Complejo R. fasciata, lo que apoya el reconocimiento de R. loeffleri como una nueva especie. Además, se reconoce a Rhyacophila fasciata viteceki como una especie distinta, R. viteceki (status promotus), basada en los resultados del análisis molecular.

The morphology of all postembryonic stages (larva, pupa, male, and female) of Rhyacophila macedonica Karaouzas, Valladolid, & Ibrahimi n. sp. from Greece, Republic of North Macedonia, Kosovo and Serbia was examined. Morphological data were supplemented by a molecular analysis of the mitochondrial cytochrome C oxidase subunit I (COI) and compared with samples of the nominate species Rhyacophila fasciata Hagen 1859, as well as with other species and subspecies in this group: Rhyacophila septentrionis McLachlan 1865, Rhyacophila denticulata McLachlan 1879, Rhyacophila sociata Navás 1916, Rhyacophila kykladica Malicky & Sipahiler 1993, Rhyacophila fasciata delici Kucinic & Valladolid 2020, and Rhyacophila fasciata viteceki Valladolid & Kucinic 2020. Our results revealed morphological differences between the nominate species and R. macedonica, as well as genetic differences among the taxa in the Rhyacophila fasciata Group, so we propose R. macedonica as a new species of the Group. Based on the new molecular data, we also elevate R. f. delici to the status of a distinct species, R. delici (status promotus).