1. Targeted Disruption of the Murine Homeodomain-Interacting Protein Kinase-2 Causes Growth Deficiency In Vivo and Cell Cycle Arrest In Vitro.
- Author
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Trapasso, Francesco, Aqeilan, Rami I., Iuliano, Rodolfo, Visone, Rosa, Gaudio, Eugenio, Ciuffini, Laura, Alder, Hansjuerg, Paduano, Francesco, Pierantoni, Giovanna Maria, Soddu, Silvia, Croce, Carlo M., and Fusco, Alfredo
- Subjects
PROTEIN kinases ,HOMEOBOX genes ,CELLULAR control mechanisms ,CELL cycle regulation ,CELL proliferation ,FIBROBLASTS - Abstract
The homeodomain-interacting protein kinase 2 (HIPK2) protein is a member of a recently identified family of nuclear protein kinases that are well conserved in various organisms. HIPK2 can bind to several homeotic factors and to a series of proteins involved in the regulation of cell survival and proliferation in response to morphogenetic and genotoxic signals. Here we report Hipk2-targeted disruption in mouse; Hipk2
−/− mice are viable and fertile but significantly smaller than their wild-type littermates. This feature is present at birth and retained throughout the mouse adulthood. Mouse embryo fibroblasts from Hipk2−/− mice show a reduced proliferation rate, compared to the wild-type counterparts, with accumulation in the G0/G1 phase of the cell cycle and altered levels of the cell cycle regulators cyclin D and CDK6. Restoration of wild-type HIPK2 expression in Hipk2−/− cells rescues the normal phenotype supporting a role for HIPK2 in the regulation of cell proliferation. [ABSTRACT FROM AUTHOR]- Published
- 2009
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