1. A Potential Profibrogenic Role of Biliary Epithelium-Derived Cardiotrophin-1 in Pediatric Cholestatic Liver Disease.
- Author
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Hua, Xiangwei, Shan, Yuhua, Li, Dawei, Xu, Dongwei, Zhang, Jiang, Yang, Taihua, Han, Longzhi, Shen, Conghuan, Xia, Yun, Chen, Qimin, Ma, Xiong, Zhang, Jianjun, and Xia, Qiang
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PEDIATRIC research , *CHILDREN'S health , *LIVER diseases , *ALCOHOLIC liver diseases , *CYTOKINES - Abstract
As a cytokine of the interleukin-6 family, cardiotrophin-1 (CT-1) has been shown to be an important endogenous protector in liver injury. Our study aimed to investigate the role of CT-1 in liver fibrosis in pediatric cholestatic liver disease (PCLD). CT-1 mRNA and protein expression levels were upregulated in PCLD liver biopsy tissues compared with controls. Immunohistochemistry and confocal microscopy of liver sections showed that CT-1 was predominantly expressed by biliary epithelium cells. Serum CT-1 was elevated significantly in the children with PCLD compared with controls. Serum CT-1 levels exhibited a moderate positive correlation with the Scheuer stage of hepatic fibrosis and serum TB levels and a weak correlation with serum ALP levels. In vitro analysis indicated that LX-2 cells preconditioned with CT-1 exhibited significant increments in proliferation and accumulation of extracellular matrix components, while also positively regulating the STAT3 and p38MAPK pathways. In conclusion, biliary epithelium-derived CT-1 may exert a profibrogenic potential in PCLD. [ABSTRACT FROM AUTHOR]
- Published
- 2015
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