1. Preparation of Nonhuman Primate Eyes for Histological Evaluation After Retinal Gene Transfer.
- Author
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Bell P, Yu H, Kuntz L, Ahonkhai O, Tretiakova A, Limberis MP, and Wilson JM
- Subjects
- Animals, Histocytological Preparation Techniques standards, Macaca fascicularis, Macaca mulatta, Gene Transfer Techniques, Genetic Therapy methods, Histocytological Preparation Techniques methods, Retina metabolism
- Abstract
To evaluate gene therapy for retinal disorders, appropriate models of the human eye are needed. Nonhuman primate eyes offer significant advantages over rodent eyes. However, current preparation methods have limitations. Here, a protocol is described for histological processing of nonhuman primate eyes after gene transfer. The user dissects unfixed eyes, flattens the globe parts within filter paper, and performs formalin fixation and paraffin embedding. This method obviates the need for harsh fixatives, allowing subsequent immunostaining or in situ hybridization while preserving tissue integrity for histopathological evaluation. Moreover, the straight orientation of the retinal cell layers is ideal for image analysis.
- Published
- 2018
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