18 results on '"Aldo, Brugnera Junior"'
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2. Effect of Er:YAG Laser and Association of Protocols on the Demineralized Enamel Microhardness
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Alexandre Morais, Ravana Angelini Sfalcin, Fernanda Yukie Kobayashi, Victor E. Arana-Chavez, Sandra Kalil Bussadori, Aldo Brugnera Junior, Fátima Zanin, and Sandra Kiss Moura
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Materials science ,genetic structures ,Enamel paint ,business.industry ,Dental enamel ,Fluoride varnish ,Biomedical Engineering ,Dentistry ,Lasers, Solid-State ,Laser ,Indentation hardness ,eye diseases ,law.invention ,stomatognathic diseases ,chemistry.chemical_compound ,surgical procedures, operative ,chemistry ,law ,visual_art ,visual_art.visual_art_medium ,Radiology, Nuclear Medicine and imaging ,sense organs ,business ,Fluoride ,Er:YAG laser - Abstract
Objective: The aim of this study was to analyze the microhardness of demineralized enamel following different treatments (fluoride varnish, Er:YAG laser, and Er:YAG laser associated with fluoride v...
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- 2021
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3. Longitudinal, Randomized, and Parallel Clinical Trial Comparing a Violet Light-Emitting Diodes System and In-Office Dental Bleaching: 6-Month Follow-Up
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Vanderlei Salvador Bagnato, Alessandra Cassoni, Sergio Brossi Botta, Marina Ferreira Pires Sobral, Aldo Brugnera Junior, Carlos Alberto Tenis, and Washington Steagall
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genetic structures ,business.industry ,Biomedical Engineering ,Dentistry ,Hydrogen Peroxide ,Dentin Sensitivity ,Clinical trial ,Tooth Sensitivity ,Tooth Bleaching ,Violet light ,Humans ,EMISSÃO DA LUZ ,Medicine ,Radiology, Nuclear Medicine and imaging ,sense organs ,Tooth Bleaching Agents ,business ,Follow-Up Studies ,Diode ,Month follow up - Abstract
Objective: This in vivo study compared two bleaching techniques with regard to the degree of tooth sensitivity (TS), color change, and treatment stability for a 6-month follow-up period. Materials ...
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- 2021
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4. Effect of Photobiomodulation on Salivary Cortisol, Masticatory Muscle Strength, and Clinical Signs in Children with Sleep Bruxism: A Randomized Controlled Trial
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Raquel Agnelli Mesquita-Ferrari, Monica da Consolação Canuto Salgueiro, Anna Carolina Ratto Tempestini Horliana, Andréa Oliver Gomes, Kristianne Porta Santos Fernandes, Fernanda Yukie Kobayashi, Marcela Leticia Leal Gonçalves, Sandra Kalil Bussadori, Aldo Brugnera Junior, and Lara Jansiski Motta
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Hydrocortisone ,medicine.medical_treatment ,Population ,Biomedical Engineering ,Dentistry ,Sleep Bruxism ,Bite Force ,law.invention ,Randomized controlled trial ,law ,medicine ,Humans ,Radiology, Nuclear Medicine and imaging ,Low-Level Light Therapy ,Child ,education ,Salivary cortisol ,education.field_of_study ,business.industry ,Gold standard ,stomatognathic diseases ,Masticatory Muscles ,Splint (medicine) ,business ,Masticatory muscle - Abstract
Background: The diagnosis of sleep bruxism (SB) in children is difficult due to the lack of a polysomnographic protocol for this population. Moreover, the gold standard treatment [occlusal splint (...
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- 2021
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5. A Randomized Comparative Clinical Study to Evaluate the Longevity of Esthetic Results of Gingival Melanin Depigmentation Treatment Using Different Laser Wavelengths (Diode, CO2, and Er:YAG)
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Marwan El Mobadder, Elie Maalouf, Samir Nammour, Melanie Namour, Toni Zeinoun, Ana Paula Brugnera, Paolo Vescovi, Eric Rompen, Amaury Namour, and Aldo Brugnera Junior
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medicine.medical_specialty ,animal structures ,Co2 laser ,integumentary system ,business.industry ,Biomedical Engineering ,Laser ,Dermatology ,Hyperpigmentation ,law.invention ,Melanin ,Clinical study ,Depigmentation ,law ,medicine ,Radiology, Nuclear Medicine and imaging ,sense organs ,medicine.symptom ,business ,After treatment ,Er:YAG laser - Abstract
Background: Gingival melanin hyperpigmentation is due to excessive deposition of melanin granules. The duration of pigmentation reappearance after treatment using different laser wavelengths remain...
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- 2020
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6. Clinical Evaluation of In-Office Dental Bleaching Using a Violet Light-Emitted Diode
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Samir Nammour, Fátima Zanin, Ana Paula Brugnera, Patricia Moreira de Freitas, Aldo Brugnera Junior, José Augusto Rodrigues, and Eric Mayer-Santos
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Adult ,Male ,Materials science ,genetic structures ,Ultraviolet Rays ,Biomedical Engineering ,Dentistry ,Carbamide Peroxide ,stomatognathic system ,Tooth Bleaching ,Humans ,Radiology, Nuclear Medicine and imaging ,Tooth Bleaching Agents ,Diode ,Tooth whitening ,business.industry ,Dentin Sensitivity ,stomatognathic diseases ,Dental Offices ,Violet light ,Tooth color ,Female ,sense organs ,Carbamide peroxide ,business ,Clinical evaluation - Abstract
Objective: To clinically evaluate the effect of the violet light-emitted diode (LED) light (405–410 nm) associated to in-office dental bleaching with 35% carbamide peroxide, on tooth color change a...
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- 2020
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7. Laser Basic Research, Clinical Use, and Innovative Teachings in Odontology
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Samir Nammour, Aldo Brugnera Junior, and Toni Zeinoun
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Engineering ,business.industry ,Basic research ,law ,Lasers ,Biomedical Engineering ,Radiology, Nuclear Medicine and imaging ,Engineering ethics ,business ,Laser ,law.invention - Published
- 2021
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8. Effect of Surface Treatment with CO2 Laser on Bond Strength in Composite Resin Restorations
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Ricardo Sgura, André Guaraci de Vito Moraes, Sandra Kalil Bussadori, Luiz Henrique Cabral Oliveira, Paulo Francisco Cesar, Aldo Brugnera Junior, Pedro H. Oliveira, Marcela Leticia Leal Gonçalves, and Márcia Regina Cabral
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Co2 laser ,Materials science ,Bond strength ,medicine.medical_treatment ,Composite number ,Resin restorations ,technology, industry, and agriculture ,Biomedical Engineering ,Laser ,law.invention ,law ,medicine ,Radiology, Nuclear Medicine and imaging ,Adhesive ,Composite material ,Dental restoration - Abstract
Objective: Evaluate the bond strength of repairs made on composite resin following the treatment of the surface of the flaw with different bonding agents and/or CO2 laser. Background: The ...
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- 2019
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9. Antimicrobial Photodynamic Therapy for the Treatment of Periodontitis and Peri-Implantitis: What Are We Missing?
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Aldo Brugnera Junior, Jamil Awad Shibli, and Letícia Helena Theodoro
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Periodontitis ,Peri-implantitis ,business.industry ,medicine.medical_treatment ,Biomedical Engineering ,Dentistry ,Photodynamic therapy ,Antimicrobial ,medicine.disease ,Peri-Implantitis ,Anti-Infective Agents ,Photochemotherapy ,medicine ,Humans ,Radiology, Nuclear Medicine and imaging ,business - Published
- 2021
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10. Biophotonics in Health Care and Its Relevance in Fighting the Coronavirus Disease
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Fatima A. A. Zanin, Samir Nammour, Sonia Groisman, and Aldo Brugnera Junior
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medicine.medical_specialty ,2019-20 coronavirus outbreak ,Coronavirus disease 2019 (COVID-19) ,Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) ,Pneumonia, Viral ,Biomedical Engineering ,Disease ,medicine.disease_cause ,Betacoronavirus ,Pandemic ,Health care ,Humans ,Medicine ,Radiology, Nuclear Medicine and imaging ,Intensive care medicine ,Pandemics ,Letter to the Editor ,Coronavirus ,SARS-CoV-2 ,business.industry ,COVID-19 ,Radiology Nuclear Medicine and imaging ,Spike Glycoprotein, Coronavirus ,Coronavirus Infections ,business - Published
- 2020
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11. Photobiomodulation Therapy in Bone Repair Associated with Bone Morphogenetic Proteins and Guided Bone Regeneration: A Histomorphometric Study
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José Alcides Almeida de Arruda, Natália Costa Brasilino, Vanda Sanderana Macêdo Carneiro, Marleny Elizabeth Martinez Gerbi, Antonio Luiz Barbosa Pinheiro, Rebeca Ferraz de Menezes, Jéssica Meirinhos Miranda, Lara Marques Magalhães Moreno, and Aldo Brugnera Junior
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Male ,Pathology ,medicine.medical_specialty ,Bone Regeneration ,Biomedical Engineering ,Bone healing ,Bone morphogenetic protein ,Random Allocation ,030207 dermatology & venereal diseases ,03 medical and health sciences ,0302 clinical medicine ,medicine ,Animals ,Radiology, Nuclear Medicine and imaging ,Femur ,Low-Level Light Therapy ,Rats, Wistar ,Bone regeneration ,Guided Tissue Regeneration ,business.industry ,Membranes, Artificial ,Surgical wound ,030206 dentistry ,Rats ,Bone Morphogenetic Proteins ,Models, Animal ,Cattle ,Lasers, Semiconductor ,business - Abstract
To evaluate the efficacy of photobiomodulation for bone repair of critical surgical wounds with implants of bone morphogenetic proteins (BMPs) and bovine biological membranes, using histological and histomorphometric analysis.Tissue engineering has been developing rapidly through the use of various biomaterials for the treatment of bone defects, such as mechanical barriers consisting of biological membranes and implants of biomaterials for bone supply.Thirty-two male rats were divided into four groups (n = 8): group I-C: control; group II-PT: photobiomodulation therapy; group III-PM: Gen-ProHistological analysis confirmed the histomorphometric results, with the experimental groups showing bone neoformation of significantly higher quality and quantity at the end of 30 days compared with the control group.Photobiomodulation therapy was effective for bone repair mainly when associated with BMPs and a biological membrane. The results of this study are promising and stimulate further scientific and clinical research.
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- 2018
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12. Histological and Thermometric Examination of Soft Tissue De-Epithelialization Using Digitally Controlled Er:YAG Laser Handpiece: An Ex Vivo Study
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Marzena Dominiak, Kinga Grzech-Leśniak, Kamil Jurczyszyn, Umberto Romeo, Piotr Ziółkowski, Aldo Brugnera Junior, and Jacek Matys
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Materials science ,Swine ,Gingiva ,Biomedical Engineering ,Lasers, Solid-State ,law.invention ,Tissue Culture Techniques ,030207 dermatology & venereal diseases ,03 medical and health sciences ,0302 clinical medicine ,Re-Epithelialization ,law ,Animals ,Radiology, Nuclear Medicine and imaging ,Mouth Mucosa ,temperature ,Soft tissue ,030206 dentistry ,Laser ,Er:YAG laser ,epithelium ,thermocouple ,vaporization ,human activities ,Ex vivo ,Biomedical engineering - Abstract
The purpose of this study was histological and thermometric examination of soft tissue de-epithelialization using digitally controlled laser handpiece (DCLH) - X-Runner.Commonly used techniques for de-epithelialization include scalpel, abrasion with diamond bur, or a combination of the two. Despite being simple, inexpensive and effective, these techniques are invasive and may produce unwanted side effects. It is important to look for alternative techniques using novel tools, which are minimally invasive and effective.114 porcine samples sized 6 × 6 mm were collected from the attached gingiva (AG) of the alveolar process of the mandible using 15C scalpel blade. The samples were irradiated by means of Er:YAG laser (LightWalker, Fotona, Slovenia), using X-Runner and HODCLH has induced significantly lower temperature increase compared with HOEr:YAG laser with DCLH handpiece may be an efficient tool in epithelium removal without excessive thermal damage.
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- 2018
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13. Hemolasertherapy: A Novel Procedure for Gingival Papilla Regeneration—Case Report
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Ana Clara Fagundes Pedroni, Fatima A. A. Zanin, Márcia Martins Marques, Aldo Brugnera Junior, MC Windlin, Maria Stella Moreira, and Ana Paula Brugnera
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Adult ,Male ,Gingival papilla ,Gingiva ,Biomedical Engineering ,Dentistry ,030207 dermatology & venereal diseases ,03 medical and health sciences ,0302 clinical medicine ,stomatognathic system ,Humans ,Regeneration ,Medicine ,Radiology, Nuclear Medicine and imaging ,Low-Level Light Therapy ,Interdental papilla ,Anterior teeth ,business.industry ,Gingival tissue ,Regeneration (biology) ,030206 dentistry ,Periodontology ,Middle Aged ,Dental Veneers ,stomatognathic diseases ,medicine.anatomical_structure ,Female ,Lasers, Semiconductor ,business - Abstract
Interdental papilla is of major importance to patients' orofacial aesthetics, especially regarding anterior teeth as part of the smile's harmony. Loss of gingival tissue, which constitutes interdental papilla, forms what in odontology is called black spaces. This loss, besides affecting the smile's aesthetics, also provokes phonetic and functional damage.The objective of the authors is to present the result of three clinical cases treated with an innovative technique called hemolasertherapy, which stimulates growth of gingival papilla and thus permanently fills in the black spaces.The photobiomodulation therapy (PBMT) used a 660 nm diode laser (Laser Duo, MMO-São Carlos, SP, Brazil), punctual, contact mode in two steps: before the bleeding (first PBMT) and immediately after bleeding (second PBMT). Parameters used were power output: 100 mW, CW; diameter tip: 5 mm; spot area: 0.19 cmThis initial study demonstrated that the blood clot originated from the bleeding provoked in the gingival area is rich in mesenchymal stem cells. PBMT enables preservation, viability, and further differentiation, stimulating the return of gingival stem cells, which would support their survival and differentiation in the blood clot, thus favoring interdental papilla regeneration.Follow-up was done for a time span of 4-5 years and considered excellent with regard to papilla preservation.
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- 2018
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14. Infrared Laser Improves Collagen Organization in Muscle and Tendon Tissue During the Process of Compensatory Overload
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Sandra Kalil Bussadori, Raquel Agnelli Mesquita Ferrari, Aldo Brugnera Junior, Daniela de Fátima Teixeira da Silva, Eric Moreno Ramos Magalhães, Kristianne Porta Santos Fernandes, and Stella Maris Lins Terena
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Male ,medicine.medical_treatment ,Biomedical Engineering ,Tendon tissue ,Muscle hypertrophy ,Tendons ,030207 dermatology & venereal diseases ,03 medical and health sciences ,0302 clinical medicine ,Laser therapy ,Animals ,Medicine ,Radiology, Nuclear Medicine and imaging ,Low-Level Light Therapy ,Rats, Wistar ,Muscle, Skeletal ,Process (anatomy) ,Low level laser therapy ,Wound Healing ,business.industry ,Far-infrared laser ,Skeletal muscle ,030206 dentistry ,Rats ,Disease Models, Animal ,medicine.anatomical_structure ,Collagen ,business ,Biomedical engineering - Abstract
The photobiomodulation using the low-level laser therapy (LLLT) exerts a positive modulating effect on the synthesis of collagen in skeletal muscles and tendons. However, few studies have addressed this effect during the compensatory overload.Evaluate the effect of infrared laser on the deposition and organization of collagen fibers in muscle and tendon tissue during compensatory overload of the plantar muscle in rats.Wistar rats were submitted to bilateral ablation of the synergist muscles of the hind paws and divided in groups: Control, Hypertrophy, and Hypertrophy (H)+LLLT (780 nm, 40 mW, 9.6 J/cmAfter 7 days an increase was observed in the area between beam muscles in H+LLLT (25.45% ± 2.56) in comparison to H (20.3% ± 3.31), in mature fibers and fibrilis in H+LLLT (29346.88 μmInfrared laser irradiation induces an improvement in collagen organization in tendons and a reduction in the total area of collagen in muscles during compensatory atrophy following the ablation of synergist muscles.
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- 2018
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15. Evaluation of the Effect of Phototherapy in Patients with Mandibular Fracture on Mandibular Dynamics, Pain, Edema, and Bite Force: A Pilot Study
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Sandra Kalil Bussadori, João Gualberto de Cerqueira Luz, Aldo Brugnera Junior, Kristianne Porta Santos Fernandes, Alessandro Melo Deana, Leandro Lauriti, Raquel Agnelli Mesquita-Ferrari, Anna Carolina Ratto Tempestini Horliana, and Larissa Costa-Santos
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Adult ,Male ,Facial swelling ,Adolescent ,Mandibular fracture ,Biomedical Engineering ,Dentistry ,Pilot Projects ,Risk Assessment ,Bite Force ,Cohort Studies ,Fracture Fixation, Internal ,Young Adult ,030207 dermatology & venereal diseases ,03 medical and health sciences ,0302 clinical medicine ,Mandibular Fractures ,Edema ,medicine ,Humans ,Radiology, Nuclear Medicine and imaging ,In patient ,Low-Level Light Therapy ,Surgical treatment ,Fracture Healing ,Pain, Postoperative ,Muscle fatigue ,business.industry ,030206 dentistry ,Phototherapy ,Prognosis ,medicine.disease ,Bite force quotient ,stomatognathic diseases ,Treatment Outcome ,Female ,medicine.symptom ,business - Abstract
The aim of this study was to evaluate the effect of phototherapy on bite force, facial swelling, mandibular movements, and pain in patients having undergone surgical treatment for mandibular fractures.These are among the predominant types of facial fractures, and treatment involving surgical fixation with titanium plates is one of the most common procedures in oral-maxillofacial surgery. Phototherapy has been used to accelerate the muscle healing process and significantly improves muscle regeneration by inducing the formation of new muscle fibers.The patients were divided into two groups: Group 1-active phototherapy, and Group 2-sham phototherapy. Both groups underwent the surgical procedure by the same surgeon using the same surgical technique. Dosimetric parameters are wavelength, 660 nm; power, 108 mW; radiant energy, 21.6 J; fluency, 21.6 J/cmThe primary variable was bite force measured with a gnathodynamometer and the secondary variables were facial swelling, mandibular movements (measured with digital calipers), and pain. The Student's t-test was used to determine intergroup differences.The findings suggest improvements in the laser group in comparison with the sham group with regard to mandibular dynamics, a reduction in postoperative facial swelling, a reduction in pain, and an increase in bite force.
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- 2018
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16. Clinical Comparison of Two Photosensitizers for Oral Cavity Decontamination
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Clovis Grecco, Hérica Adad Ricci Donato, Sebastião Pratavieira, Vanderlei Salvador Bagnato, Cristina Kurachi, Aldo Brugnera-Junior, Universidade Estadual Paulista (Unesp), Universidade de São Paulo (USP), and Camilo Castelo Branco University
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0301 basic medicine ,030103 biophysics ,Saliva ,Curcumin ,curcuminoids ,hematoporphyrin ,Biomedical Engineering ,Dentistry ,Context (language use) ,Pharmacology ,Oral cavity ,Oral hygiene ,clinical ,photodynamic inactivation ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,In vivo ,Humans ,Medicine ,Radiology, Nuclear Medicine and imaging ,Decontamination ,Hematoporphyrin ,Mouth ,Photosensitizing Agents ,business.industry ,030206 dentistry ,Human decontamination ,Antimicrobial ,Healthy Volunteers ,MICROBIOLOGIA ORAL ,Hematoporphyrins ,in vivo ,Treatment Outcome ,Photochemotherapy ,chemistry ,business - Abstract
Made available in DSpace on 2018-12-11T16:45:46Z (GMT). No. of bitstreams: 0 Previous issue date: 2017-02-01 Objective: In this study, we aim to compare the photodynamic inactivation (PDI) effects of two different photosensitizers (PS), Photogem® and Natural Curcumin, irradiated with light-emitted diodes (LED) at 630 and 450 nm, respectively. Background: The current antimicrobial mouthwash for oral hygiene has several drawbacks. In this context, PDI is an alternative technique to inactivate pathogenic microbes in mucosa and in periodontal tissue. Furthermore, there are numerous infectious diseases that may affect the oral cavity, motivating the use of PDI in dentistry. Methods: The volunteers (n = 50) were randomize separated into five experimental groups (n = 5) for each PS: water control, PS control, light control, and two PS concentrations (25 and 100 mg/L). Each patient underwent mouthwash solution containing the PS before illumination procedure that was performed with an LED device. For microbial decontamination evaluation, the saliva was collected three times: before (T0), immediately after (T1), and 24 h after the illumination procedure (T2). After that, the difference between the colony forming units (CFU) for each volunteer was compared. Results: The results show that regardless of PS and treatment applied, there was microbial reduction immediately after PDI, however, after 24 h only Natural Curcumin still presents a reduction. For Photogem after 24 h, the microorganism returns to the original CFU. Conclusions: Immediately after PDI, both PS have the same efficiency, nevertheless the Natural Curcumin still has an efficacy after 24 h and also is a more viable photosensitizer. In addition, the results indicate that PDI can be a promised technique used for microbial reducing for the oral cavity. School of Dentistry of Araraquara UNESP University of Estadual Paulista São Carlos Institute of Physics University of São Paulo, PO Box 369 Biomedical Engineer Research Center CEB Camilo Castelo Branco University School of Dentistry of Araraquara UNESP University of Estadual Paulista
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- 2017
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17. Effects of Treatment for Manipulation of Teeth and Er:YAG Laser Irradiation on Dentin: A Raman Spectroscopy Analysis
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Airton Abrahão Martin, Aldo Brugnera Junior, Marcos Tadeu Tavares Pacheco, Luís Eduardo Silva Soares, and Fatima A. A. Zanin
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Molar ,Materials science ,Biomedical Engineering ,Analytical chemistry ,Spectrum Analysis, Raman ,law.invention ,Tissue Culture Techniques ,chemistry.chemical_compound ,symbols.namesake ,stomatognathic system ,law ,Dentin ,medicine ,Humans ,Radiology, Nuclear Medicine and imaging ,Irradiation ,Spectroscopy ,Phosphoric acid ,Lasers ,Laser ,medicine.anatomical_structure ,chemistry ,Dental Etching ,symbols ,Molar, Third ,Raman spectroscopy ,Er:YAG laser ,Nuclear chemistry - Abstract
The main purpose of this study was to evaluate the utility of Raman spectroscopy analysis as a research tool to study the effects of Er:YAG laser etching on dentin mineral and organic components. A secondary aim was to study the effects of the decontamination process and the storage procedure on dentin components.There are no spectroscopy reports relating the effects of Er:YAG laser irradiation as an alternative to acid etching and the manipulation process on the dentin structure.Twelve non-carious human third molars were divided in two main groups: stored in thymol solution (group A, n = 6) or autoclaved (group B, n = 6). The specimens were either etched with 37% phosphoric acid (control subgroup) or irradiated with Er:YAG laser. Irradiated samples were divided into the following subgroups: I, II, and III (80 mJ, 3 Hz, 30 sec; 120 mJ, 3 Hz, 30 sec; and 180 mJ, 3 Hz, 30 sec, respectively). Samples were analyzed by Raman spectroscopy.The mineral and organic dentin contents were more affected in autoclaved teeth than in the specimens stored in thymol. Peak area reduction in group A specimens treated with phosphoric acid and pulse energy of 80 mJ were the most conservative surface treatments regarding changes in the peak area of organic and inorganic dentin components.The autoclaving process and pulse energies of 120 and 180 mJ produced greater reduction of organic and inorganic contents in dentin, associated with greater reduction in the areas of 968, 1077, 1460, and 1670 cm(1) Raman peaks.
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- 2007
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18. Disinfection of Root Canals Using Er:YAG Laser at Different Frequencies
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Rosimeire Pietro, Márcia Saran Vezzani, Yara Teresinha Corrêa Silva-Sousa, Manoel Damião Sousa-Neto, and Aldo Brugnera-Junior
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Staphylococcus aureus ,Veterinary medicine ,Biomedical Engineering ,Dentistry ,In Vitro Techniques ,Bacterial growth ,Enterococcus faecalis ,chemistry.chemical_compound ,Candida albicans ,Humans ,Radiology, Nuclear Medicine and imaging ,biology ,business.industry ,Chemistry ,Pipette ,biology.organism_classification ,Disinfection ,Pseudomonas aeruginosa ,Brain heart infusion ,Laser Therapy ,sense organs ,Dental Pulp Cavity ,business ,Root Canal Preparation ,After treatment ,Er:YAG laser ,Bacillus subtilis - Abstract
This study evaluated, in vitro, the degree of disinfection of the Er:YAG laser in root canals contaminated with Bacillus subtilis, Staphylococcus aureus, Enterococcus faecalis, Pseudomonas aeruginosa, and Candida albicans, for 28 days.Forty-six single-rooted human teeth were divided into five groups of eight teeth each; three teeth were used as negative controls and three as positive controls. After contamination, the root canals were prepared mechanically. Three groups were irradiated with Er:YAG laser at 100 mJ, varying the frequency (7, 10, and 16 Hz). Two groups were irrigated with 1.0% and 2.5% NaOCl solution. After treatment, two sterilized paper cones were placed in the root canals for 5 min. One cone was transferred to 2.0 mL of Letheen broth culture medium, incubated at 37 degrees C for 48 h, and then 0.1 mL of that solution was placed in 2.0 mL of brain heart infusion for 48 h to determine microbial growth. The other cone was transferred to a test pipette with peptone and water for serial dilution and spread in Müeller Hinton medium. After 24 h of incubation, the colony-forming units (CFUs) were counted.There was a microbial reduction of 85.33% for the group irradiated with Er:YAG laser at 100 mJ/7 Hz, 74.58% at 100 mJ/10 Hz, and 89.50% at 100 mJ/16 Hz. For the groups irrigated with 1.0% and 2.5% NaOCl solution, 83.15% and 84.46% values of microbial reduction were obtained respectively.All the groups showed statistically similar results (p0.05%). No method totally eliminated microorganisms.
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- 2006
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